• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

巴马小型猪鼻腔的组织学和解剖结构

Histological and anatomical structure of the nasal cavity of Bama minipigs.

作者信息

Yang Jingjing, Dai Lei, Yu Qinghua, Yang Qian

机构信息

Veterinary College, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, PR China.

出版信息

PLoS One. 2017 Mar 24;12(3):e0173902. doi: 10.1371/journal.pone.0173902. eCollection 2017.

DOI:10.1371/journal.pone.0173902
PMID:28339502
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5365122/
Abstract

OBJECTIVE

The nasal mucosa is equipped with abundant lymphatic tissues, serving as the first line of defense against invasion by microorganisms. In this study, we characterized the features of the nasal mucosa of Bama minipigs (Sus scrofa domestica) via histological analysis.

METHODS

Five cross sections (I, II, III, IV, and V) were obtained from the distal end of the nasal cavity toward the pharynx (along the cavity axis) and examined. Specifically, CD3+ T cells, immunoglobulin A (IgA)+ cells, and M cells were detected by immunohistochemistry, while dendritic cells (DCs) were detected by immunofluorescence. The distribution of goblet cells was determined by periodic acid-Schiff (PAS) staining.

RESULTS

The nasal cavity of Bama minipigs can be divided into three parts: the regio vestibularis (I, II), regio respiratoria (III, IV), and regio olfactoria (V). Lymphoid tissue was present at random locations in the nasal cavity. Abundant lymphoid tissue was located in the roof of the nasopharyngeal meatus and was continuous with the lymphoid tissue of the pharynx. The distribution of CD3+ T cells, IgA+ cells, M cells, and DCs increased distally in the nasal cavity.

CONCLUSIONS

The present work comprises a histological study of the nasal cavity of Bama minipigs, and will be beneficial for understanding the mechanisms of immunity in these animals after nasal vaccination.

摘要

目的

鼻黏膜配备有丰富的淋巴组织，作为抵御微生物入侵的第一道防线。在本研究中，我们通过组织学分析对巴马小型猪（Sus scrofa domestica）鼻黏膜的特征进行了表征。

方法

从鼻腔远端向咽部（沿腔轴）获取五个横截面（I、II、III、IV和V）并进行检查。具体而言，通过免疫组织化学检测CD3 + T细胞、免疫球蛋白A（IgA）+细胞和M细胞，而通过免疫荧光检测树突状细胞（DC）。杯状细胞的分布通过过碘酸希夫（PAS）染色确定。

结果

巴马小型猪的鼻腔可分为三个部分：前庭区（I、II）、呼吸区（III、IV）和嗅区（V）。淋巴组织随机分布于鼻腔中。丰富的淋巴组织位于鼻咽道顶部，并与咽部的淋巴组织相连。CD3 + T细胞、IgA +细胞、M细胞和DC的分布在鼻腔远端增加。

结论

本研究是对巴马小型猪鼻腔的组织学研究，将有助于理解这些动物鼻内接种疫苗后的免疫机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/c0fbc3154c5e/pone.0173902.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/b9567eeae09e/pone.0173902.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/a80dec83ce70/pone.0173902.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/05a3f4382661/pone.0173902.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/0268f6bc97b4/pone.0173902.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/3ad78a57efe7/pone.0173902.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/676292eac532/pone.0173902.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/71d81ffbeb93/pone.0173902.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/a0e3ba29ceb4/pone.0173902.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/c0fbc3154c5e/pone.0173902.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/b9567eeae09e/pone.0173902.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/a80dec83ce70/pone.0173902.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/05a3f4382661/pone.0173902.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/0268f6bc97b4/pone.0173902.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/3ad78a57efe7/pone.0173902.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/676292eac532/pone.0173902.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/71d81ffbeb93/pone.0173902.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/a0e3ba29ceb4/pone.0173902.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e640/5365122/c0fbc3154c5e/pone.0173902.g009.jpg

相似文献

1
Histological and anatomical structure of the nasal cavity of Bama minipigs.巴马小型猪鼻腔的组织学和解剖结构
PLoS One. 2017 Mar 24;12(3):e0173902. doi: 10.1371/journal.pone.0173902. eCollection 2017.
2
Characterization of nasal cavity-associated lymphoid tissue in ducks.鸭鼻腔相关淋巴组织的特征。
Anat Rec (Hoboken). 2014 May;297(5):916-24. doi: 10.1002/ar.22888. Epub 2014 Mar 3.
3
NALT (nasal cavity-associated lymphoid tissue) in the rabbit.兔子的鼻腔相关淋巴组织（NALT）
Vet Immunol Immunopathol. 2010 Feb 15;133(2-4):212-8. doi: 10.1016/j.vetimm.2009.08.011. Epub 2009 Aug 19.
4
Nasal passages of Göttingen minipigs from the neonatal period to young adult.从新生期到青年期的哥廷根小型猪的鼻腔
Toxicol Pathol. 2012 Jun;40(4):656-66. doi: 10.1177/0192623311436175. Epub 2012 Feb 1.
5
Immunohistochemistry of the nasal cavity-associated lymphoid tissue in the dolphin (Stenella coeruleoalba, Meyen 1833).海豚（Stenella coeruleoalba，Meyen 1833）鼻腔相关淋巴组织的免疫组织化学。
Microsc Res Tech. 2024 Sep;87(9):2103-2112. doi: 10.1002/jemt.24592. Epub 2024 Apr 29.
6
Histological studies on the development of porcine tonsils after birth.出生后猪扁桃体发育的组织学研究。
J Morphol. 2018 Aug;279(8):1185-1193. doi: 10.1002/jmor.20839. Epub 2018 Jun 11.
7
Light microscopic, electron microscopic, and immunohistochemical comparison of Bama minipig (Sus scrofa domestica) and human skin.巴马小型猪（Sus scrofa domestica）与人类皮肤的光学显微镜、电子显微镜及免疫组织化学比较
Comp Med. 2010 Apr;60(2):142-8.
8
Distribution of T-cell subsets and immunoglobulin-containing cells in nasal-associated lymphoid tissue (NALT) of chickens.鸡鼻相关淋巴组织（NALT）中T细胞亚群和含免疫球蛋白细胞的分布。
Histol Histopathol. 2000 Jul;15(3):713-20. doi: 10.14670/HH-15.713.
9
[Comparative macro- and microscopic anatomy of the nasal cavity of European insectivores].[欧洲食虫目动物鼻腔的比较宏观与微观解剖学]
Gegenbaurs Morphol Jahrb. 1975;121(6):698-756.
10
Delivery of antigen to nasal-associated lymphoid tissue microfold cells through secretory IgA targeting local dendritic cells confers protective immunity.通过分泌型 IgA 靶向局部树突状细胞将抗原递送至鼻相关淋巴组织微褶细胞可赋予保护性免疫。
J Allergy Clin Immunol. 2016 Jan;137(1):214-222.e2. doi: 10.1016/j.jaci.2015.07.042. Epub 2015 Sep 26.

引用本文的文献

1
Respiratory transmission potential of severe fever with thrombocytopenia syndrome bunyavirus: evidence from intranasal exposure in a humanized mouse model.发热伴血小板减少综合征布尼亚病毒的呼吸道传播潜力：来自人源化小鼠模型鼻内暴露的证据
Emerg Microbes Infect. 2025 Dec;14(1):2511134. doi: 10.1080/22221751.2025.2511134. Epub 2025 Jun 19.
2
Influenza A Virus Utilizes the Nasolacrimal System to Establish Respiratory Infection after Ocular Exposure in the Swine Model.甲型流感病毒利用鼻泪系统在猪模型的眼部暴露后建立呼吸道感染。
Transbound Emerg Dis. 2024 Jun 27;2024:8192499. doi: 10.1155/2024/8192499. eCollection 2024.
3

本文引用的文献

1
Comparative efficacy of intranasal and oral vaccines against Bordetella bronchiseptica in dogs.犬用鼻内疫苗和口服疫苗针对支气管败血波氏杆菌的比较疗效
Vet J. 2016 Jun;212:71-7. doi: 10.1016/j.tvjl.2016.04.004. Epub 2016 Apr 12.
2
Dendritic Cells and Monocytes with Distinct Inflammatory Responses Reside in Lung Mucosa of Healthy Humans.具有不同炎症反应的树突状细胞和单核细胞存在于健康人类的肺黏膜中。
J Immunol. 2016 Jun 1;196(11):4498-509. doi: 10.4049/jimmunol.1600071. Epub 2016 May 2.
3
TLR4 and TLR7/8 Adjuvant Combinations Generate Different Vaccine Antigen-Specific Immune Outcomes in Minipigs when Administered via the ID or IN Routes.
Single-cell analysis of nasal epithelial cell development in domestic pigs.
家猪鼻上皮细胞发育的单细胞分析。
Vet Res. 2024 Oct 30;55(1):140. doi: 10.1186/s13567-024-01403-w.
4
Development of a New Swine Model Resembling Human Empty Nose Syndrome.开发一种类似人类空鼻综合征的新型猪模型。
Medicina (Kaunas). 2024 Sep 24;60(10):1559. doi: 10.3390/medicina60101559.
5
Chitosan-Based Thermogelling System for Nose-to-Brain Donepezil Delivery: Optimising Formulation Properties and Nasal Deposition Profile.用于鼻脑靶向递送多奈哌齐的壳聚糖基热凝胶系统：优化制剂性质和鼻腔沉积概况
Pharmaceutics. 2023 Jun 5;15(6):1660. doi: 10.3390/pharmaceutics15061660.
6
Characteristics of the nasal mucosa of commercial pigs during normal development.商业猪正常发育过程中鼻腔黏膜的特征。
Vet Res. 2023 Apr 24;54(1):37. doi: 10.1186/s13567-023-01164-y.
7
Mucosal Vaccination Against Periodontal Disease: Current Status and Opportunities.黏膜免疫接种防治牙周病：现状与机遇。
Front Immunol. 2021 Dec 2;12:768397. doi: 10.3389/fimmu.2021.768397. eCollection 2021.
8
Dietary Betaine Addition Alters Carcass Traits, Meat Quality, and Nitrogen Metabolism of Bama Mini-Pigs.添加膳食甜菜碱可改变巴马小型猪的胴体性状、肉质和氮代谢。
Front Nutr. 2021 Aug 27;8:728477. doi: 10.3389/fnut.2021.728477. eCollection 2021.
9
A Dry Powder Platform for Nose-to-Brain Delivery of Dexamethasone: Formulation Development and Nasal Deposition Studies.一种用于地塞米松鼻脑递送的干粉平台：制剂开发与鼻腔沉积研究
Pharmaceutics. 2021 May 26;13(6):795. doi: 10.3390/pharmaceutics13060795.
10
Maternal Probiotic or Synbiotic Supplementation Modulates Jejunal and Colonic Antioxidant Capacity, Mitochondrial Function, and Microbial Abundance in Bama Mini-piglets.母体补充益生菌或合生元可调节巴马仔猪空肠和结肠的抗氧化能力、线粒体功能及微生物丰度。
Oxid Med Cell Longev. 2021 May 4;2021:6618874. doi: 10.1155/2021/6618874. eCollection 2021.
当通过皮内或鼻内途径给药时，TLR4与TLR7/8佐剂组合在小型猪中产生不同的疫苗抗原特异性免疫结果。
PLoS One. 2016 Feb 10;11(2):e0148984. doi: 10.1371/journal.pone.0148984. eCollection 2016.
4
Respiratory nanoparticle-based vaccines and challenges associated with animal models and translation.基于呼吸道纳米颗粒的疫苗以及与动物模型和转化相关的挑战。
J Control Release. 2015 Dec 10;219:622-631. doi: 10.1016/j.jconrel.2015.09.047. Epub 2015 Sep 26.
5
Goblet Cell Derived RELM-β Recruits CD4+ T Cells during Infectious Colitis to Promote Protective Intestinal Epithelial Cell Proliferation.杯状细胞衍生的RELM-β在感染性结肠炎期间募集CD4+ T细胞，以促进保护性肠上皮细胞增殖。
PLoS Pathog. 2015 Aug 18;11(8):e1005108. doi: 10.1371/journal.ppat.1005108. eCollection 2015 Aug.
6
Intranasal Immunization with Pressure Inactivated Avian Influenza Elicits Cellular and Humoral Responses in Mice.用压力灭活禽流感进行鼻内免疫可在小鼠中引发细胞和体液反应。
PLoS One. 2015 Jun 9;10(6):e0128785. doi: 10.1371/journal.pone.0128785. eCollection 2015.
7
A bama minipig model of laryngopharyngeal reflux and the change of laryngopharyngeal mucosal ultrastructure.巴马小型猪喉咽反流模型及喉咽黏膜超微结构的变化
J Neurogastroenterol Motil. 2015 Mar 30;21(2):182-8. doi: 10.5056/jnm14113.
8
H9N2 influenza whole inactivated virus combined with polyethyleneimine strongly enhances mucosal and systemic immunity after intranasal immunization in mice.H9N2流感全灭活病毒与聚乙烯亚胺联合使用，在小鼠鼻内免疫后能显著增强黏膜免疫和全身免疫。
Clin Vaccine Immunol. 2015 Apr;22(4):421-9. doi: 10.1128/CVI.00778-14. Epub 2015 Feb 11.
9
Evaluation of change in the skin concentration of tazarotene and betamethasone dipropionate based on drug-drug interaction for transdermal drug delivery in miniature pig.基于药物相互作用评估微型猪经皮给药时他扎罗汀和丙酸倍氯米松皮肤浓度的变化。
Xenobiotica. 2015 May;45(5):380-4. doi: 10.3109/00498254.2014.985762. Epub 2014 Nov 20.
10
An experimental model for Staphylococcus aureus hepatic abscess in Bama minipig.巴马小型猪金黄色葡萄球菌肝脓肿实验模型
Genet Mol Res. 2014 Feb 21;13(3):7113-22. doi: 10.4238/2014.February.21.12.