Dey Shatovisha, Krishna Sriram, Anthony Nicholas B, Rhoads Douglas D
Program in Cell and Molecular Biology, University of Arkansas, Fayetteville, AR 72701.
Department of Biological Sciences, University of Arkansas, Fayetteville, AR 72701.
Poult Sci. 2017 Apr 1;96(4):788-797. doi: 10.3382/ps/pew380.
Previously, we reported a genome wide association study (GWAS) that had shown association of a region between 11.8 and 13.6 Mbp on chromosome 9 with ascites phenotype in broilers. We had used microsatellite loci to demonstrate an association of particular genotypes for this region with ascites in experimental ascites lines and commercial broiler breeder lines. We identified two potential candidate genes, AGTR1 and UTS2D, within that chromosomal region for mediating the quantitative effect. We have now extended our analysis using SNPs for these genes to assess association with resistance or susceptibility to ascites in these same broiler lines. Surprisingly, in contrast to our previous GWAS and microsatellite data for this region, we find no association of the SNP genotypes or haplotypes in the region suggesting that the two genes might have limited association with the disease phenotype.
此前，我们报道了一项全基因组关联研究（GWAS），该研究表明9号染色体上11.8至13.6兆碱基对之间的一个区域与肉鸡腹水表型相关。我们使用微卫星位点来证明该区域的特定基因型与实验性腹水品系和商业肉鸡育种品系中的腹水相关。我们在该染色体区域内鉴定出两个潜在的候选基因AGTR1和UTS2D，用于介导数量效应。我们现在使用这些基因的单核苷酸多态性（SNP）扩展了分析，以评估与这些相同肉鸡品系中腹水抗性或易感性的关联。令人惊讶的是，与我们之前针对该区域的GWAS和微卫星数据相反，我们发现该区域的SNP基因型或单倍型没有关联，这表明这两个基因可能与疾病表型的关联有限。
