Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland.
Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland.
Poult Sci. 2017 Jul 1;96(7):2091-2097. doi: 10.3382/ps/pex030.
The available literature lacks information on the effect of Zakarpacki zeolite (clinoptilolite) on the immune system of poultry. Therefore, the aim of this study was to determine the effect of this zeolite on selected indicators of the immune response in poultry by evaluating the expression of cluster of differentiation (CD) surface molecules on T and B lymphocytes and the concentration of IL-2 and IL-10 in the blood. Ninety one-day-old Ross 308 broiler chicks were used in the study. The birds were divided into 3 groups of 30 each. The same basic diet was used in all groups, but groups II and III received a feed additive in the form of 2% and 3% zeolite. Blood samples were collected from all birds on the 40th day of observations. Weight gain in the birds in both experimental groups was significantly higher, and no clinical symptoms of disease were observed. The percentage of CD4+CD25+ T and B lymphocytes was higher in both groups receiving zeolite, but the percentage of CD8+CD25+ T lymphocytes was higher only in the group receiving 3% zeolite. There were no differences between the groups in the percentage of cells with CD3+ and MHC Class II expression. Higher serum concentrations of IL-2 and IL-10 were noted only in group III. The use of zeolites enhances antigen presentation and leads to increased Th1 and Th2 response. Excessive supply of zeolite in the feed leads to a local inflammatory response, which may cause damage to the intestinal barrier.
关于扎卡尔帕cki 沸石（斜发沸石）对家禽免疫系统影响的现有文献资料较少。因此，本研究旨在通过评估 T 和 B 淋巴细胞表面分化群（CD）分子的表达以及血液中 IL-2 和 IL-10 的浓度，来确定这种沸石对家禽某些免疫反应指标的影响。本研究选用 91 日龄罗斯 308 肉鸡 90 只，随机分为 3 组，每组 30 只。所有组均使用相同的基础日粮，但第 2 组和第 3 组分别添加 2%和 3%沸石作为饲料添加剂。所有鸡在观察第 40 天采集血样。两个实验组的鸡体重增加明显高于对照组，且未观察到任何疾病临床症状。两组中 CD4+CD25+T 和 B 淋巴细胞的比例均较高，但仅在添加 3%沸石的组中 CD8+CD25+T 淋巴细胞的比例较高。各组之间 CD3+和 MHC Class II 表达细胞的比例没有差异。仅在第 3 组中观察到血清中 IL-2 和 IL-10 浓度升高。沸石的使用增强了抗原呈递，导致 Th1 和 Th2 反应增加。饲料中沸石过量供应会导致局部炎症反应，从而可能破坏肠道屏障。
