棓酸癸酯对巴西副球孢子菌gp43诱导A549肺细胞凋亡的抗凋亡作用。

Anti-apoptotic effects of decyl gallate on the induction of apoptosis in A549 pneumocytes by Paracoccidioides brasiliensis gp43.

作者信息

Bernardi Thais, da Silva Julhiany de Fátima, Vicentin Juliana, de Oliveira Haroldo Cesar, Assato Patricia Akemi, Marcos Caroline Maria, de Paula E Silva Ana Carolina Alves, da Silva Rosangela Aparecida Moraes, Regasini Luis Octávio, Silva Dulce Helena Siqueira, da Silva Bolzani Vanderlan, Fusco-Almeida Ana Marisa, Mendes-Giannini Maria José Soares

机构信息

Universidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Campus Araraquara, Departamento de Análises Clínicas, Laboratório de Micologia Clínica, Araraquara, São Paulo, Brasil.

Universidade Estadual Paulista (UNESP), Instituto de Biociências, Letras e Ciências Exatas, Campus São José do Rio Preto, Departamento de Química e Ciências Ambientais, São José do Rio Preto, São Paulo, Brasil.

出版信息

Med Mycol. 2017 Nov 1;55(8):890-894. doi: 10.1093/mmy/myx013.

DOI:10.1093/mmy/myx013

PMID:28339963

Abstract

Apoptosis is considered an escape mechanism from the host immune system for the fungus Paracoccidioides spp, and it serves as a vehicle for entry into macrophages without stimulating microbicidal activities. Recently, gp43 of P. brasiliensis was demonstrated to be involved in this process. Therefore, as a new therapeutic alternative, it is very important to study compounds that could reduce the modulation of the induction of apoptosis caused by this fungus. Decyl gallate (G14) is a known antifungal compound, and we decided to investigate its anti-apoptotic properties. Our results demonstrate that G14 was effective against apoptosis induced by gp43, as observed in epithelial cells, and led to a reduction in DNA damage, Bak down-regulation and Bcl-2 up-regulation. Together, these data show that G14 presents promising anti-apoptotic activity.

摘要

细胞凋亡被认为是巴西副球孢子菌逃避宿主免疫系统的一种机制，它作为一种进入巨噬细胞的载体，却不会刺激杀菌活性。最近，巴西副球孢子菌的gp43被证明参与了这一过程。因此，作为一种新的治疗选择，研究能够减少该真菌引起的细胞凋亡诱导调节的化合物非常重要。没食子酸癸酯（G14）是一种已知的抗真菌化合物，我们决定研究其抗凋亡特性。我们的结果表明，G14对gp43诱导的上皮细胞凋亡有效，并导致DNA损伤减少、Bak下调和Bcl-2上调。总之，这些数据表明G14具有有前景的抗凋亡活性。