Nunes C F, Setotaw T A, Pasqual M, Chagas E A, Santos E G, Santos D N, Lima C G B, Cançado G M A
Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, Montes Claros, MG, Brasil
Departamento de Agricultura, Universidade Federal de Lavras, Lavras, MG, Brasil.
Genet Mol Res. 2017 Mar 22;16(1):gmr-16-01-gmr.16019409. doi: 10.4238/gmr16019409.
Myrciaria dubia (camu-camu) is an Amazon tree that produces a tart fruit with high vitamin C content. It is probably the fruit with the highest vitamin C content among all Brazilian fruit crops and it can be used to supplement daily vitamin C dose. This property has attracted the attention of consumers and, consequently, encouraged fruit farmers to produce it. In order to identify and select potential accessions for commercial exploitation and breeding programs, M. dubia has received considerable research attention. The identification and characterization of genetic diversity, as well as identification of the population structure of accessions preserved in germplasm banks are fundamental for the success of any breeding program. The objective of this study was to evaluate the genetic variability of 10 M. dubia populations obtained from the shores of Reis Lake, located in the municipality of Caracaraí, Roraima, Brazil. Fourteen polymorphic inter simple sequence repeat (ISSR) markers were used to study the population genetic diversity, which resulted in 108 identified alleles. Among the 14 primers, GCV, UBC810, and UBC827 produced the highest number of alleles. The study illustrated the suitability and efficiency of ISSR markers to study the genetic diversity of M. dubia accessions. We also revealed the existence of high genetic variability among both accessions and populations that can be exploited in future breeding programs and conservation activities of this species.
杜氏番樱桃（卡姆果）是一种生长在亚马逊地区的树木，其果实味道酸涩，但维生素C含量很高。它可能是巴西所有水果作物中维生素C含量最高的水果，可用于补充每日维生素C摄入量。这一特性吸引了消费者的关注，从而鼓励果农进行种植。为了识别和选择具有商业开发潜力的品种以及用于育种计划，杜氏番樱桃受到了大量的研究关注。识别和表征遗传多样性，以及确定保存在种质库中的品种的种群结构，对于任何育种计划的成功都至关重要。本研究的目的是评估从巴西罗赖马州卡拉卡拉伊市雷斯湖岸边采集的10个杜氏番樱桃种群的遗传变异性。使用14个多态性简单序列重复区间（ISSR）标记来研究种群遗传多样性，共鉴定出108个等位基因。在这14个引物中，GCV、UBC810和UBC827产生的等位基因数量最多。该研究表明ISSR标记适用于研究杜氏番樱桃品种的遗传多样性，且效率较高。我们还揭示了品种和种群中都存在较高的遗传变异性，可在该物种未来的育种计划和保护活动中加以利用。
