噬菌体Mu-1整合机制：萘啶酸处理导致Mu-1诱导的突变在复制起点附近聚集。
Mechanism of phage Mu-1 integration: nalidixic acid treatment causes clustering of Mu-1-induced mutations near replication origin.
作者信息
Paolozzi L, Jucker R, Calef E
出版信息
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4940-3. doi: 10.1073/pnas.75.10.4940.
Abstract
Frequencies of Mu-1-induced mutants of Escherichia coli have been compared under two different experimental conditions: cells in exponential growth and the same cells treated with nalidixic acid. The average of values obtained from the nalidixic acid-treated culture is 3 times higher than that obtained from the control. Individual ratios of the frequency of mutants in the two cultures yield decreasing values from 6 to 1, starting from the point of origin of DNA replication to the termini of DNA replication. These results are compatible with the idea that Mu-1 integrates at the replication fork.
摘要
已在两种不同实验条件下比较了Mu - 1诱导的大肠杆菌突变体的频率：处于指数生长期的细胞和用萘啶酸处理的相同细胞。从萘啶酸处理的培养物中获得的值的平均值比从对照中获得的值高3倍。两种培养物中突变体频率的个体比率从DNA复制起点到DNA复制终点产生从6到1的递减值。这些结果与Mu - 1在复制叉处整合的观点一致。
相似文献
1
Mechanism of phage Mu-1 integration: nalidixic acid treatment causes clustering of Mu-1-induced mutations near replication origin.噬菌体Mu-1整合机制：萘啶酸处理导致Mu-1诱导的突变在复制起点附近聚集。
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4940-3. doi: 10.1073/pnas.75.10.4940.
2
State of prophage Mu DNA upon induction.诱导后原噬菌体Mu DNA的状态。
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3143-7. doi: 10.1073/pnas.74.8.3143.
3
Induction of lambda prophage by nalidixic acid.萘啶酸诱导λ原噬菌体
Virology. 1970 Jun;41(2):244-55. doi: 10.1016/0042-6822(70)90076-0.
4
Induction of phage lambda by transferred irradiated colI DNA.通过转移的经辐照的colI DNA诱导λ噬菌体
Mol Gen Genet. 1969;106(1):14-24. doi: 10.1007/BF00332817.
6
Synchronous reinitiation of chromosome replication in E. coli B-r after nalidixic acid treatment.萘啶酸处理后大肠杆菌B-r中染色体复制的同步重新起始
Proc Natl Acad Sci U S A. 1970 Jun;66(2):365-9. doi: 10.1073/pnas.66.2.365.
7
Studies on the mechanism of action of nalidixic acid.萘啶酸作用机制的研究。
Antimicrob Agents Chemother. 1973 Oct;4(4):479-86. doi: 10.1128/AAC.4.4.479.
8
9
Inhibition of bacterial segregation by early functions of phage mu and association of replication protein B with the inner cell membrane.噬菌体μ早期功能对细菌分离的抑制作用以及复制蛋白B与细菌内膜的关联
Mol Gen Genet. 1986 Mar;202(3):461-6. doi: 10.1007/BF00333277.
10
[Compatibility of transposable phages of Escherichia coli and Pseudomonas aeruginosa. I. Co-development of phages Mu and D3112 and integration of phage D3112 into RP4::Mu plasmid in Pseudomonas aeruginosa cells].[大肠杆菌和铜绿假单胞菌转座噬菌体的相容性。I. 噬菌体Mu和D3112的共同发育以及噬菌体D3112在铜绿假单胞菌细胞中整合到RP4::Mu质粒中]
Genetika. 1988 Apr;24(4):634-40.
引用本文的文献
1
Two classes of Mu lig mutants: the thermosensitives for integration and replication and the hyperproducers for ligase.两类穆氏连接酶（Mu lig）突变体：整合与复制的温度敏感型以及连接酶的高产型。
Nucleic Acids Res. 1980 Dec 11;8(23):5859-73. doi: 10.1093/nar/8.23.5859.
2
Integration of bacteriophage Mu at host chromosomal replication forks during lytic development.在裂解发育过程中噬菌体Mu在宿主染色体复制叉处的整合。
Proc Natl Acad Sci U S A. 1980 May;77(5):2801-5. doi: 10.1073/pnas.77.5.2801.
3
Escherichia coli K-12 gyrB gene product is involved in the lethal effect of the ligts2 mutant of bacteriophage Mu.大肠杆菌K-12 gyrB基因产物参与噬菌体Mu的ligts2突变体的致死效应。
J Bacteriol. 1984 Feb;157(2):665-8. doi: 10.1128/jb.157.2.665-668.1984.
4
Recombination involving transposable elements: role of target molecule replication in Tn1 delta Ap-mediated replicon fusion.涉及转座元件的重组：靶分子复制在Tn1 delta Ap介导的复制子融合中的作用。
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2314-7. doi: 10.1073/pnas.80.8.2314.
5
Conservative integration of bacteriophage Mu DNA into pBR322 plasmid.噬菌体Mu DNA保守整合到pBR322质粒中。
Proc Natl Acad Sci U S A. 1982 Jul;79(14):4362-6. doi: 10.1073/pnas.79.14.4362.
6
Host DNA replication forks are not preferred targets for bacteriophage Mu transposition.宿主DNA复制叉不是噬菌体Mu转座的首选靶点。
J Bacteriol. 1985 Jul;163(1):282-90. doi: 10.1128/jb.163.1.282-290.1985.
7
Replication forks of Escherichia coli are not the preferred sites for lysogenic integration of bacteriophage Mu.大肠杆菌的复制叉不是噬菌体Mu溶原整合的首选位点。
J Bacteriol. 1988 Jul;170(7):3089-93. doi: 10.1128/jb.170.7.3089-3093.1988.
本文引用的文献
1
Segregation of Lambda Lysogenicity during Bacterial Recombination in Escherichia Coli K12.大肠杆菌K12中细菌重组过程中λ原噬菌体溶源性的分离
Genetics. 1954 Jul;39(4):429-39. doi: 10.1093/genetics/39.4.429.
2
[Genetic study of a temperate bacteriophage of Escherichia coli. l. The genetic system of the bacteriophage].[大肠杆菌一种温和噬菌体的遗传学研究。I. 噬菌体的遗传系统]
Ann Inst Pasteur (Paris). 1954 Dec;87(6):653-73.
3
BACTERIOPHAGE-INDUCED MUTATION IN ESCHERICHIA COLI.噬菌体诱导的大肠杆菌突变
Proc Natl Acad Sci U S A. 1963 Dec;50(6):1043-51. doi: 10.1073/pnas.50.6.1043.
4
O0 and strong-polar mutations in the gal operon are insertions.O0和gal操纵子中的强极性突变是插入突变。
Mol Gen Genet. 1968;102(4):353-63. doi: 10.1007/BF00433726.
5
Insertion of phage Mu. 1 within prophage lambda. A new approach for studying the control of the late functions in bacteriophage lambda.噬菌体Mu.1插入原噬菌体λ内。一种研究噬菌体λ晚期功能调控的新方法。
Mol Gen Genet. 1969;106(1):89-92. doi: 10.1007/BF00332824.
6
Bacteriophage Mu integration: on the mechanism of Mu-induced mutations.噬菌体Mu整合：关于Mu诱导突变的机制
J Mol Biol. 1971 Nov 28;62(1):171-8. doi: 10.1016/0022-2836(71)90137-9.
7
Electron microscopic evidence for linear insertion of bacteriophage MU-1 in lysogenic bacteria.噬菌体MU-1在溶原性细菌中线性插入的电子显微镜证据。
J Virol. 1971 Oct;8(4):551-63. doi: 10.1128/JVI.8.4.551-563.1971.
8
Attachment of prophage P2: gene order at different host chromosomal sites.原噬菌体P2的附着：不同宿主染色体位点的基因顺序
Virology. 1969 Dec;39(4):867-81. doi: 10.1016/0042-6822(69)90023-3.
9
An L-arabinose binding protein and arabinose permeation in Escherichia coli.大肠杆菌中的一种L-阿拉伯糖结合蛋白与阿拉伯糖渗透作用
J Mol Biol. 1969 Nov 28;46(1):185-96. doi: 10.1016/0022-2836(69)90065-5.
10
Induction of excessive deoxyribonucleic acid synthesis in Escherichia coli by nalidixic acid.萘啶酸诱导大肠杆菌中过量的脱氧核糖核酸合成。
J Bacteriol. 1967 Nov;94(5):1664-71. doi: 10.1128/jb.94.5.1664-1671.1967.
Zotero 插件