两类穆氏连接酶（Mu lig）突变体：整合与复制的温度敏感型以及连接酶的高产型。
Two classes of Mu lig mutants: the thermosensitives for integration and replication and the hyperproducers for ligase.
作者信息
Paolozzi L, Ghelardini P, Liebart J C, Capozzoni A, Marchelli C
出版信息
Nucleic Acids Res. 1980 Dec 11;8(23):5859-73. doi: 10.1093/nar/8.23.5859.
Abstract
We have previously shown that Mu restores an active DNA replication at non-permissive temperature in E. coli K12 ligts7 strains. In this paper we describe two new types of phage mutants for the Mu lig function. The Mu ligts mutants are conditional lethals defective for both integration and replication of DNA, unable to 'complement' the bacterial lig mutation; the map between B and lys. The mutants of the other type, on the other hand, are able to restore to a maximum level the activity impaired by the ligts7 mutation in the host. We suggest the hypothesis that the product of Mu lig gene could be part of a complex as a topoisomerase.
摘要
我们之前已经表明，在大肠杆菌K12 ligts7菌株中，Mu在非允许温度下可恢复活跃的DNA复制。在本文中，我们描述了两种新型的Mu连接酶功能噬菌体突变体。Mu ligts突变体是DNA整合和复制均有缺陷的条件致死突变体，无法“互补”细菌的连接酶突变；其图谱位于B和lys之间。另一方面，另一种类型的突变体能够将宿主中因ligts7突变而受损的活性恢复到最高水平。我们提出一个假说，即Mu连接酶基因的产物可能作为拓扑异构酶成为复合体的一部分。
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Two classes of Mu lig mutants: the thermosensitives for integration and replication and the hyperproducers for ligase.两类穆氏连接酶（Mu lig）突变体：整合与复制的温度敏感型以及连接酶的高产型。
Nucleic Acids Res. 1980 Dec 11;8(23):5859-73. doi: 10.1093/nar/8.23.5859.
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Synchronous division induced in Escherichia coli K12 by gemts mutants of phage Mu.噬菌体Mu的gemts突变体在大肠杆菌K12中诱导的同步分裂。
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本文引用的文献
1
Segregation of Lambda Lysogenicity during Bacterial Recombination in Escherichia Coli K12.大肠杆菌K12中细菌重组过程中λ原噬菌体溶源性的分离
Genetics. 1954 Jul;39(4):429-39. doi: 10.1093/genetics/39.4.429.
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Transduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles.大肠杆菌和痢疾志贺氏菌菌株间乳糖利用能力的转导及转导噬菌体颗粒的特性
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3
Restoration of ligase activity in E. coli K12 lig ts7 strain by bacteriophage Mu and cloning of a DNA fragment harbouring the Mu 'lig' gene.噬菌体Mu恢复大肠杆菌K12 lig ts7菌株中的连接酶活性并克隆携带Mu“lig”基因的DNA片段。
Nucleic Acids Res. 1980 Jul 25;8(14):3157-73. doi: 10.1093/nar/8.14.3157.
4
Integration of bacteriophage Mu at host chromosomal replication forks during lytic development.在裂解发育过程中噬菌体Mu在宿主染色体复制叉处的整合。
Proc Natl Acad Sci U S A. 1980 May;77(5):2801-5. doi: 10.1073/pnas.77.5.2801.
5
Enzymatic characterization of a mutant of Escherichia coli with an altered DNA ligase.具有改变的DNA连接酶的大肠杆菌突变体的酶学特性分析。
Proc Natl Acad Sci U S A. 1971 May;68(5):1002-5. doi: 10.1073/pnas.68.5.1002.
6
Prophage deletion mapping of bacteriophage Mu-1.噬菌体Mu-1的原噬菌体缺失图谱分析
Virology. 1973 Jul;54(1):93-101. doi: 10.1016/0042-6822(73)90118-9.
7
Restoration of DNA synthesis at non-permissive temperature and of UV resistance induced by bacteriophage Mu in Escherichia coli lig ts7 strain.在大肠杆菌连接酶ts7菌株中，噬菌体Mu诱导的非允许温度下DNA合成的恢复以及紫外线抗性的恢复。
Ann Microbiol (Paris). 1979 Oct;130B(3):275-85.
8
The mechanism of integration of phage Mu in the chromosome of Escherichia coli.
Biochem Biophys Res Commun. 1979 May 14;88(1):111-6. doi: 10.1016/0006-291x(79)91703-0.
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Behavior of bacteriophage Mu DNA upon infecton of Escherichia coli cells.噬菌体Mu DNA感染大肠杆菌细胞后的行为。
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