College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Talanta. 2017 May 15;167:111-117. doi: 10.1016/j.talanta.2017.02.013. Epub 2017 Feb 4.
Visual detection of tumor markers with accuracy and selectivity exhibits significant advantages for cancer early diagnosis due to its low-cost and simple analytical instruments. Herein, a highly photosensitive colorimetric immunoassay for carcino embryonie antigen (CEA, as a model) detection based on Cu doped Ag-AgI nanocomposite was constructed. First, Cu doped Ag-AgI nanocomposite (Cu/Ag-AgI) owned the ability of oxidization of TMB was synthesized by impregnation method, which was characterized by transmission electron microscope (TEM), scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray powder diffraction (XRD), UV-Vis diffuse reflectance spectroscopy (DRS) and X-ray photoelectron spectroscopy (XPS). Second, based on sandwich-type immunoassay, the immunocomplex was built on the surface of magnetic bead by utilizing Cu/Ag-AgI as labels. The blue color of TMB was obtained under visible irradiation and ultraviolet spectrum scanning was carried out in ABS buffer solution. Under optimal conditions, the absorbance values increased with the increasing of CEA concentrations in sample with the linearity range of 1.0pgmL-5.0ngmL. The detection limit was 0.13pgmL. More importantly, the developed colorimetric immunoassay exhibited good selectivity, repeatability, stability and possible applications in the real serum sample analysis.
由于其低成本和简单的分析仪器，具有准确性和选择性的肿瘤标志物的可视化检测在癌症早期诊断方面具有显著优势。在此，构建了基于掺杂 Cu 的 Ag-AgI 纳米复合材料的癌胚抗原（CEA，作为模型）检测的高灵敏度比色免疫测定法。首先，通过浸渍法合成了具有 TMB 氧化能力的掺杂 Cu 的 Ag-AgI 纳米复合材料（Cu/Ag-AgI），通过透射电子显微镜（TEM）、扫描电子显微镜（SEM）、能量色散 X 射线光谱（EDX）、X 射线粉末衍射（XRD）、紫外可见漫反射光谱（DRS）和 X 射线光电子能谱（XPS）对其进行了表征。其次，基于三明治型免疫测定法，利用 Cu/Ag-AgI 作为标记物在磁性珠表面构建免疫复合物。在可见光照射下获得 TMB 的蓝色，在 ABS 缓冲溶液中进行紫外光谱扫描。在最佳条件下，吸光度值随样品中 CEA 浓度的增加而增加，线性范围为 1.0pgmL-5.0ngmL。检测限为 0.13pgmL。更重要的是，所开发的比色免疫测定法表现出良好的选择性、重复性、稳定性，并且可用于实际血清样品分析。
