College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.
Mikrochim Acta. 2024 Aug 22;191(9):555. doi: 10.1007/s00604-024-06630-8.
A novel signal amplification strategy was developed by combining near-infrared light with MoS/CuO/Au nanocomposite for building a colorimetric immunoassay. First, MoS/CuO/Au nanocomposite was synthesized by precipitation and photoreduction methods and characterized by scanning electron microscopy (SEM) and X-ray powder diffraction (XRD). MoS/CuO/Au nanocomposite has oxidase-like activity and can oxidize TMB to form a blue product (TMBox). Further, the catalytic oxidation of TMB was accelerated under near-infrared (NIR) laser radiation. The sandwich-type colorimetric immunoassay was constructed using MoS/CuO/Au nanocomposite. Under the enhancement of near-infrared light, carcinoembryonic antigen (CEA) was sensitively detected in the range 0.1 to 40 ng/mL with the limit of detection of 0.03 ng/mL. Moreover, the immunosensor has excellent selectivity and anti-interference, good repeatability, and stability.
一种新型的信号放大策略是通过将近红外光与 MoS/CuO/Au 纳米复合材料结合起来，构建比色免疫分析。首先，通过沉淀和光还原法合成了 MoS/CuO/Au 纳米复合材料，并通过扫描电子显微镜 (SEM) 和 X 射线粉末衍射 (XRD) 进行了表征。MoS/CuO/Au 纳米复合材料具有氧化酶样活性，可以将 TMB 氧化形成蓝色产物 (TMBox)。此外，在近红外 (NIR) 激光辐射下，TMB 的催化氧化加速。利用 MoS/CuO/Au 纳米复合材料构建三明治型比色免疫分析。在近红外光的增强下，癌胚抗原 (CEA) 在 0.1 至 40 ng/mL 的范围内被灵敏地检测到，检测限为 0.03 ng/mL。此外，该免疫传感器具有优异的选择性和抗干扰性、良好的重复性和稳定性。
