BİBAM (Plant, Drug and Scientific Researches Center), Anadolu University, Eskişehir, Turkey.
Department of Chemistry, Anadolu University, Eskişehir, Turkey.
Talanta. 2017 May 15;167:172-180. doi: 10.1016/j.talanta.2017.01.093. Epub 2017 Feb 4.
Cancer antigen 125 (CA 125) is widely used as diagnostic biomarker for ovarian cancer. Change in the concentration level of CA 125 is associated with disease progression or regression. CA 125 posseses a phosphorylation site and protein backbone is phosphorylated on serine, before secretion. In this study, we have developed an imprinting method for CA 125 recognition and determination. In this method, methacryloyl antipyrine europium (III) [(MAAP)-Eu(III)] and methacryloyl antipyrine terbium (III) [(MAAP)-Tb(III)] have been used as new metal-chelating monomers via metal coordination-chelation interactions. Phosphoserine (PS) has been used as a template for the detection of CA 125. PS imprinted carbon nanotube (CNT) and FeO nanoparticle (SPN) have cavities that are selective for CA 125. The binding affinity of the PS imprinted CNT and SPN nanosensor has been investigated using Langmuir adsorption isotherms and affinity constants (K) have found to be 1.85 10M for PS and 13.5 10 mLU and 7.73 10 mLU for CA 125 (for CNT and SPN, respectively). Detection limit of PS imprinted CNT nanosensor for PS and CA 125 have been found to be 1.77 10M and 0.49 UmL, respectively. Human serum samples have been spiked with different concentrations of CA 125 (in pH 7.4 PBS) to investigate the feasibility of the nanosensors for clinical applications. Experimental results have been revealed that prepared nanosensors have been exhibited better sensivity, recovery and reproducibility.
癌抗原 125（CA 125）被广泛用作卵巢癌的诊断生物标志物。CA 125 浓度的变化与疾病的进展或消退有关。CA 125 具有一个磷酸化位点，在分泌前，其蛋白骨架在丝氨酸上发生磷酸化。在本研究中，我们开发了一种用于 CA 125 识别和测定的印迹方法。在该方法中，通过金属配位-螯合相互作用，将丙烯酰基安替比林铕（III）[(MAAP)-Eu(III)]和丙烯酰基安替比林铽（III）[(MAAP)-Tb(III)]用作新的金属螯合单体。磷酸丝氨酸（PS）被用作检测 CA 125 的模板。PS 印迹的碳纳米管（CNT）和 FeO 纳米颗粒（SPN）具有对 CA 125 具有选择性的空腔。通过 Langmuir 吸附等温线研究了 PS 印迹的 CNT 和 SPN 纳米传感器的结合亲和力，并且发现 PS 印迹的 CNT 和 SPN 的亲和常数（K）分别为 1.85 10M 和 13.5 10 mLU 和 7.73 10 mLU。PS 印迹的 CNT 纳米传感器对 PS 和 CA 125 的检测限分别为 1.77 10M 和 0.49 UmL。向人血清样品中加入不同浓度的 CA 125（在 pH 7.4 PBS 中），以研究纳米传感器用于临床应用的可行性。实验结果表明，所制备的纳米传感器具有更好的灵敏度、回收率和重现性。
