Gültekin Aytaç, Karanfil Gamze, Kuş Mahmut, Sönmezoğlu Savaş, Say Rıdvan
Department of Energy Systems Engineering, Faculty of Engineering, Karamanoğlu Mehmetbey University, Karaman 70200, Turkey.
Department of Energy Systems Engineering, Faculty of Engineering, Karamanoğlu Mehmetbey University, Karaman 70200, Turkey.
Talanta. 2014 Feb;119:533-7. doi: 10.1016/j.talanta.2013.11.053. Epub 2013 Nov 28.
In the present work, a new caffeic acid imprinted quartz crystal microbalance (QCM) nanosensor has been designed for selective assignation of caffeic acid in plant materials. Methacrylamidoantipyrine-iron(III) [MAAP-Fe(III)] as metal-chelating monomer has been used to prepare selective molecular imprinted polymer (MIP). MIP film for detection of caffeic acid has been developed on QCM electrode and selectivity experiments and analytical performance of caffeic acid imprinted QCM nanosensor has been studied. The caffeic acid imprinted QCM nanosensor has been characterized by AFM. After the characterization studies, imprinted and non-imprinted nanosensors was connected to QCM system for studies of connection of the target molecule, selectivity and the detection of amount of target molecule in real samples. The detection limit was found to be 7.8 nM. The value of Langmuir constant (b) (4.06 × 10(6)) that was acquired using Langmuir graph demonstrated that the affinity of binding sites was strong. Also, selectivity of prepared caffeic acid imprinted nanosensor was found as being high compared to chlorogenic acid. Finally, the caffeic acid levels in plant materials was determined by the prepared QCM nanosensor.
在本工作中，设计了一种新型的咖啡酸印迹石英晶体微天平（QCM）纳米传感器，用于选择性测定植物材料中的咖啡酸。采用甲基丙烯酰胺基安替比林 - 铁（III）[MAAP - Fe（III）]作为金属螯合单体来制备选择性分子印迹聚合物（MIP）。在QCM电极上制备了用于检测咖啡酸的MIP膜，并研究了咖啡酸印迹QCM纳米传感器的选择性实验和分析性能。通过原子力显微镜（AFM）对咖啡酸印迹QCM纳米传感器进行了表征。在表征研究之后，将印迹和非印迹纳米传感器连接到QCM系统，用于研究目标分子的结合、选择性以及实际样品中目标分子含量的检测。检测限为7.8 nM。使用朗缪尔图获得的朗缪尔常数（b）（4.06×10⁶）值表明结合位点的亲和力很强。此外，发现所制备的咖啡酸印迹纳米传感器与绿原酸相比具有较高的选择性。最后，用所制备的QCM纳米传感器测定了植物材料中的咖啡酸含量。
