ICBAS/UP - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua Jorge Viterbo Ferreira 228, P 4050-313 Porto, Portugal; CIIMAR/CIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Av. General Norton de Matos, 4050-208 Matosinhos, Portugal.
ICBAS/UP - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua Jorge Viterbo Ferreira 228, P 4050-313 Porto, Portugal; Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Porto, Portugal.
Talanta. 2017 May 15;167:688-694. doi: 10.1016/j.talanta.2017.03.017. Epub 2017 Mar 6.
In this work, a potentiometric flow injection method is described for the fast bi-parametric determination of iodide and iodate in urine and salt samples. The developed methodology aimed for iodine speciation with a potentially portable system (running on batteries). The iodate reduction to iodide was effectively attained in line within the same manifold. The iodide determination was accomplished in the dynamic range of 2.50×10-1.00×10M and the total iodine dynamic range, resulted from iodide plus iodate, was 3.50×10-2.00×10M. The calculated limits of detection were 1.39×10M and 1.77×10M for iodide and iodate, respectively. A determination rate of 21h for the bi-parametric iodide and iodate determination was obtained for sample injection. The urine samples (RSD <5.8% for iodide and RSD <7.0% for iodate) results were in agreement with those obtained by the classic Sandell-Kolthoff reaction colorimetric reference procedure (RD <7.0%) and standard samples from Centers for Disease Control and Prevention, Atlanta, USA (CDC) international inter-laboratory EQUIP program. The developed flow method was also successfully applied to the iodide and iodate determination in salt samples (RSD <3.1% for iodate and iodide), with comparable results to conventional procedures. No significant interferences were observed interference percentage <9% for both determinations.
在这项工作中，描述了一种电位流动注射法，用于快速双参数测定尿液和盐样中的碘化物和碘酸盐。所开发的方法旨在实现碘形态的分析，具有潜在的便携系统（使用电池运行）。在同一个流路中有效地实现了将碘酸盐还原为碘化物。碘化物的测定范围为 2.50×10-1.00×10M，总碘的测定范围为 3.50×10-2.00×10M，结果为碘化物加碘酸盐。碘化物和碘酸盐的计算检出限分别为 1.39×10M 和 1.77×10M。对于样品注射，双参数碘化物和碘酸盐的测定速率为 21h。尿液样品（碘化物的 RSD <5.8%，碘酸盐的 RSD <7.0%）的结果与经典的 Sandell-Kolthoff 反应比色参考程序（RD <7.0%）和美国疾病控制与预防中心（CDC）国际实验室 EQUIP 计划的标准样品获得的结果一致。所开发的流动方法也成功地应用于盐样中碘化物和碘酸盐的测定（碘酸盐的 RSD <3.1%，碘化物的 RSD <3.1%），结果与常规方法相当。对于两种测定，未观察到显著干扰（干扰百分比 <9%）。
