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在蒙古临床健康的小反刍动物中发现的假定新型血清型“33”和“35”扩展了非典型 BTV 群。

Putative Novel Serotypes '33' and '35' in Clinically Healthy Small Ruminants in Mongolia Expand the Group of Atypical BTV.

机构信息

Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, 17943 Greifswald-Insel Riems, Germany.

School of Veterinary Medicine, Mongolian University of Life Sciences, Khan-uul District, Zaisan 17024, Mongolia.

出版信息

Viruses. 2020 Dec 29;13(1):42. doi: 10.3390/v13010042.

DOI:10.3390/v13010042
PMID:33383902
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7824028/
Abstract

Between 2015 and 2018, we identified the presence of three so-far-unknown Bluetongue virus (BTV) strains (BTV-MNG1/2018, BTV-MNG2/2016, and BTV-MNG3/2016) circulating in clinical healthy sheep and goats in Mongolia. Virus isolation from EDTA blood samples of BTV-MNG1/2018 and BTV-MNG3/2016 was successful on the mammalian cell line BSR using blood collected from surveillance. After experimental inoculation of goats with BTV-MNG2/2016 positive blood as inoculum, we observed viraemia in one goat and with the EDTA blood of the experimental inoculation, the propagation of BTV-MNG2/2016 in cell culture was successful on mammalian cell line BSR as well. However, virus isolation experiments for BTV-MNG2/2016 on KC cells were unsuccessful. Furthermore, we generated the complete coding sequence of all three novel Mongolian strains. For atypical BTV, serotyping via the traditional serum neutralization assay is not trivial. We therefore sorted the 'putative novel atypical serotypes' according to their segment-2 sequence identities and their time point of sampling. Hence, the BTV-MNG1/2018 isolate forms the 'putative novel atypical serotype' 33, the BTV-MNG3/2016 the 'putative novel atypical serotype' 35, whereas the BTV-MNG2/2016 strain belongs to the same putative novel atypical serotype '30' as BTV-XJ1407 from China.

摘要

在 2015 年至 2018 年期间，我们在蒙古的临床健康绵羊和山羊中发现了三种迄今为止未知的蓝舌病毒（BTV）株（BTV-MNG1/2018、BTV-MNG2/2016 和 BTV-MNG3/2016）。使用从监测中收集的 EDTA 血液样本从 BTV-MNG1/2018 和 BTV-MNG3/2016 成功分离病毒。在使用 BTV-MNG2/2016 阳性血液作为接种物对山羊进行实验接种后，我们在一只山羊中观察到病毒血症，并且在用 EDTA 血液进行实验接种后，BTV-MNG2/2016 在哺乳动物细胞系 BSR 中的增殖也获得成功。然而，BTV-MNG2/2016 的病毒分离实验在 KC 细胞上未能成功。此外，我们生成了所有三种新型蒙古株的完整编码序列。对于非典型 BTV，通过传统的血清中和试验进行血清型鉴定并不简单。因此，我们根据它们的节段 2 序列同一性及其采样时间点对“推定的新型非典型血清型”进行了分类。因此，BTV-MNG1/2018 分离株形成“推定的新型非典型血清型”33，BTV-MNG3/2016 形成“推定的新型非典型血清型”35，而 BTV-MNG2/2016 菌株与来自中国的 BTV-XJ1407 属于同一推定的新型非典型血清型“30”。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55a3/7824028/15d553f114c9/viruses-13-00042-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55a3/7824028/eb18efb4cbe8/viruses-13-00042-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55a3/7824028/b3ce954c4594/viruses-13-00042-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55a3/7824028/15d553f114c9/viruses-13-00042-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55a3/7824028/eb18efb4cbe8/viruses-13-00042-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55a3/7824028/b3ce954c4594/viruses-13-00042-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55a3/7824028/15d553f114c9/viruses-13-00042-g003a.jpg

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