Department of Cell and Molecular Pharmacology & Experimental Therapeutics, Medical University of South Carolina, Charleston, USA; Medical University of South Carolina Proteomics Center, Medical University of South Carolina, Charleston, USA.
Department of Pediatrics and Cell Development and Biology, Vanderbilt University Medical Center, Nashville, TN, USA.
Biochim Biophys Acta Proteins Proteom. 2017 Jul;1865(7):927-935. doi: 10.1016/j.bbapap.2017.03.009. Epub 2017 Mar 21.
Significant progress has been made for tissue imaging of proteins using matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI IMS). These advancements now facilitate mapping of a wide range of proteins, peptides, and post-translational modifications in a wide variety of tissues; however, the use of MALDI IMS to detect proteins from cardiac tissue is limited. This review discusses the most recent advances in protein imaging and demonstrates application to cardiac tissue, including the heart valve. Protein imaging by MALDI IMS allows multiplexed histological mapping of proteins and protein components that are inaccessible by antibodies and should be considered an important tool for basic and clinical cardiovascular research. This article is part of a Special Issue entitled: MALDI Imaging, edited by Dr. Corinna Henkel and Prof. Peter Hoffmann.
利用基质辅助激光解吸电离成像质谱（MALDI IMS）对蛋白质进行组织成像已取得重大进展。这些进展现在促进了在广泛的组织中对各种蛋白质、肽和翻译后修饰的映射；然而，MALDI IMS 用于检测心脏组织中的蛋白质的应用受到限制。本综述讨论了蛋白质成像的最新进展，并展示了其在心脏组织中的应用，包括心脏瓣膜。MALDI IMS 的蛋白质成像允许通过抗体无法访问的蛋白质和蛋白质成分的多重组织学映射，应被视为基础和临床心血管研究的重要工具。本文是由 Corinna Henkel 博士和 Peter Hoffmann 教授编辑的特刊：MALDI 成像的一部分。
