Ma Shuai, Xu Qingqing, Deng Bo, Zheng Yin, Tian Hongyan, Wang Li, Ding Feng
Division of Nephrology & Unit of Critical Nephrology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, 639 Zhizaoju Road, Shanghai, 200011, China.
Division of Nephrology, Huashan Hospital, Fudan University, 12 Wulumuqi Zhong Road, Shanghai, 200040, China.
Intensive Care Med Exp. 2017 Dec;5(1):18. doi: 10.1186/s40635-017-0129-2. Epub 2017 Mar 24.
Overwhelming activation of granulocytes and monocytes is central to inflammatory responses during sepsis. Granulocyte and monocyte adsorptive apheresis (GMA) is an extracorporeal leukocyte apheresis device filled with cellulose acetate beads and selectively adsorbs granulocytes and monocytes from the peripheral blood.
In this study, septic rats received the GMA treatment for 2 h at 18 h after cecal ligation and puncture.
GMA selectively adsorbed activated neutrophils and monocytes from the peripheral blood, reduced serum inflammatory cytokine expression, and seemed to improve organ injuries and animal survival. GMA potentially reduced lung injury by alleviating the infiltration of inflammatory cells and the secretion of cytokines.
This study showed that selective granulocyte and monocyte adsorption with cellulose acetate beads might ameliorate cecal ligation and puncture (CLP)-induced sepsis and improve survival and organ function.
在脓毒症期间，粒细胞和单核细胞的过度激活是炎症反应的核心。粒细胞和单核细胞吸附性血液成分分离术（GMA）是一种体外白细胞去除装置，其填充有醋酸纤维素珠，可从外周血中选择性吸附粒细胞和单核细胞。
在本研究中，脓毒症大鼠在盲肠结扎和穿刺后18小时接受GMA治疗2小时。
GMA从外周血中选择性吸附活化的中性粒细胞和单核细胞，降低血清炎症细胞因子表达，似乎改善了器官损伤和动物存活率。GMA可能通过减轻炎症细胞浸润和细胞因子分泌来减轻肺损伤。
本研究表明，用醋酸纤维素珠选择性吸附粒细胞和单核细胞可能改善盲肠结扎和穿刺（CLP）诱导的脓毒症，并改善存活率和器官功能。
