School of Integrative Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Republic of Korea.
School of Integrative Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Republic of Korea.
Biosens Bioelectron. 2017 Aug 15;94:485-499. doi: 10.1016/j.bios.2017.03.032. Epub 2017 Mar 18.
A novel strategy to precisely detect or monitor various biomaterials in living cells poses paramount importance in understanding cellular processes. Graphene, a newly emerged two-dimensional carbon material, has been widely utilized for biosensors owing to its multifarious characteristics including mechanical, electrical, and optical properties (e.g. stability, conductivity, fluorescence quenching and photoluminescence). In addition, graphene derivatives and their innate characteristics, such as biocompatibility low cytotoxicity and water solubility have facilitated the use of graphene-based materials for live cell biosensing, wherein graphene is utilized as a core material by itself or in combination with other functional nanomaterials to load target-specific probes, fluorescent dyes, and other signaling molecules. Such graphene-based hybrid nanomaterials have been employed to detect various cellular entities in living cells, including ions, biomolecules, genetic molecules, proteins, enzymes, and even whole cells. The following review will discuss a number of previous studies in which graphene-based hybrid constructs were used for live cell biosensing, and their potential applications in cancer research and stem cell therapy.
一种精确检测或监测活细胞中各种生物材料的新策略对于理解细胞过程至关重要。石墨烯作为一种新兴的二维碳材料，由于其具有机械、电气和光学特性（例如稳定性、导电性、荧光猝灭和光致发光），已被广泛应用于生物传感器。此外，石墨烯衍生物及其固有特性，如生物相容性、低细胞毒性和水溶性，促进了基于石墨烯的材料在活细胞生物传感中的应用，其中石墨烯本身或与其他功能纳米材料结合用作核心材料来负载针对特定目标的探针、荧光染料和其他信号分子。这些基于石墨烯的杂化纳米材料已被用于检测活细胞中的各种细胞实体，包括离子、生物分子、遗传分子、蛋白质、酶，甚至整个细胞。以下综述将讨论一些之前的研究，这些研究使用了基于石墨烯的杂化构建体进行活细胞生物传感，以及它们在癌症研究和干细胞治疗中的潜在应用。
