School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China; Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA.
Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA.
Bioresour Technol. 2017 Jun;234:389-396. doi: 10.1016/j.biortech.2017.03.073. Epub 2017 Mar 15.
Clostridium tyrobutyricum can utilize glucose and xylose as carbon source for butyric acid production. However, xylose catabolism is inhibited by glucose, hampering butyric acid production from lignocellulosic biomass hydrolysates containing both glucose and xylose. In this study, an engineered strain of C. tyrobutyricum Ct-pTBA overexpressing heterologous xylose catabolism genes (xylT, xylA, and xylB) was investigated for co-utilizing glucose and xylose present in hydrolysates of plant biomass, including soybean hull, corn fiber, wheat straw, rice straw, and sugarcane bagasse. Compared to the wild-type strain, Ct-pTBA showed higher xylose utilization without significant glucose catabolite repression, achieving near 100% utilization of glucose and xylose present in lignocellulosic biomass hydrolysates in bioreactor at pH 6. About 42.6g/L butyrate at a productivity of 0.56g/L·h and yield of 0.36g/g was obtained in batch fermentation, demonstrating the potential of C. tyrobutyricum Ct-pTBA for butyric acid production from lignocellulosic biomass hydrolysates.
丁酸梭菌可以利用葡萄糖和木糖作为碳源生产丁酸。然而，葡萄糖会抑制木糖的代谢，从而阻碍了木质纤维素生物质水解液中同时含有葡萄糖和木糖时丁酸的生产。在这项研究中，研究了一株过表达异源木糖代谢基因（xylT、xylA 和 xylB）的工程化丁酸梭菌 Ct-pTBA，用于共利用植物生物质水解液中的葡萄糖和木糖，包括大豆皮、玉米纤维、小麦秸秆、水稻秸秆和甘蔗渣。与野生型菌株相比，Ct-pTBA 表现出更高的木糖利用率，而没有明显的葡萄糖分解代谢物抑制，在 pH 值为 6 的生物反应器中几乎 100%地利用了木质纤维素生物质水解液中的葡萄糖和木糖。在分批发酵中，获得了 42.6g/L 的丁酸，生产力为 0.56g/L·h，产率为 0.36g/g，表明丁酸梭菌 Ct-pTBA 有潜力从木质纤维素生物质水解液中生产丁酸。
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