South China University of Technology, Guangzhou, China.
Appl Biochem Biotechnol. 2010 Jan;160(2):350-9. doi: 10.1007/s12010-008-8305-1. Epub 2008 Jul 24.
Butyric acid has many applications in chemical, food, and pharmaceutical industries. In the present study, Clostridium tyrobutyricum ATCC 25755 was immobilized in a fibrous-bed bioreactor to evaluate the performance of butyrate production from glucose and xylose. The results showed that the final concentration and yield of butyric acid were 13.70 and 0.46 g g(-1), respectively, in batch fermentation when 30 g L(-1) glucose was introduced into the bioreactor. Furthermore, high concentration 10.10 g L(-1) and yield 0.40 g g(-1) of butyric acid were obtained with 25 g L(-1) xylose as the carbon source. The immobilized cells of C. tyrobutyricum ensured similar productivity and yield from repeated batch fermentation. In the fed-batch fermentation, the final concentration of butyric acid was further improved to 24.88 g L(-1) with one suitable glucose feeding in the fibrous-bed bioreactor. C. tyrobutyricum immobilized in the fibrous-bed bioreactor would provide an economically viable fermentation process to convert the reducing sugars derived from plant biomass into the final bulk chemical (butyric acid).
丁酸在化学、食品和制药等行业有广泛的应用。本研究利用纤维床生物反应器固定化酪丁酸梭菌 ATCC 25755，考察了以葡萄糖和木糖为碳源生产丁酸的性能。结果表明，当 30 g/L 葡萄糖被引入生物反应器时，分批发酵的最终丁酸浓度和产率分别达到 13.70 和 0.46 g/g。此外，当以 25 g/L 木糖为碳源时，丁酸的浓度和产率分别高达 10.10 g/L 和 0.40 g/g。固定化酪丁酸梭菌细胞保证了重复分批发酵过程具有相似的生产力和产率。在补料分批发酵中，通过在纤维床生物反应器中进行一次合适的葡萄糖补料，丁酸的最终浓度进一步提高到 24.88 g/L。在纤维床生物反应器中固定化的酪丁酸梭菌为利用植物生物质衍生的还原糖转化为最终大宗化学品（丁酸）提供了一种经济可行的发酵工艺。
