InVita Healthcare Technologies, Jacksonville Beach, FL, USA.
Methods Mol Biol. 2023;2685:253-262. doi: 10.1007/978-1-0716-3295-6_16.
The Investigator 24Plex kits are multiplex PCR kits utilized by forensic laboratories to simultaneously amplify 22 of the most commonly utilized STR markers for human identity testing, including the 20 core CODIS loci, along with the sex marker Amelogenin and 2 novel quality sensors. These quality sensors are unique internal PCR controls that provide useful insight to the analyst regarding possible inhibition or degradation within the sample. This chapter describes the use of the QS version of the kit designed for use with extracted DNA from casework samples, as well as the use of the GO! version of the kit designed for direct amplification of reference samples.
Investigator 24Plex 试剂盒是法医实验室使用的多重 PCR 试剂盒，用于同时扩增人类身份检测中最常用的 22 个 STR 标记物，包括 20 个核心 CODIS 基因座，以及性别标记物 Amelogenin 和 2 个新型质量传感器。这些质量传感器是独特的内部 PCR 控制物，为分析师提供有关样品中可能存在的抑制或降解的有用信息。本章介绍了用于从案件样本中提取 DNA 的 QS 版试剂盒的使用方法，以及用于直接扩增参比样本的 GO! 版试剂盒的使用方法。
