Mounce Bryan C, Cesaro Teresa, Carrau Lucia, Vallet Thomas, Vignuzzi Marco
Institut Pasteur, Viral Populations and Pathogenesis Unit, CNRS UMR 3569, 25-28 rue du Dr. Roux, Paris, France.
Institut Pasteur, Viral Populations and Pathogenesis Unit, CNRS UMR 3569, 25-28 rue du Dr. Roux, Paris, France; University of Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France.
Antiviral Res. 2017 Jun;142:148-157. doi: 10.1016/j.antiviral.2017.03.014. Epub 2017 Mar 24.
Several compounds extracted from spices and herbs exhibit antiviral effects in vitro, suggesting potential pharmacological uses. Curcumin, a component of turmeric, has been used as a food additive and herbal supplement due to its potential medicinal properties. Previously, curcumin exhibited antiviral properties against several viruses, including dengue virus and hepatitis C virus, among others. Here, we describe the antiviral effect of curcumin on Zika and chikungunya viruses, two mosquito-borne outbreak viruses. Both viruses responded to treatment of cells with up to 5 μM curumin without impacting cellular viability. We observed that direct treatment of virus with curcumin reduced infectivity of virus in a dose- and time-dependent manner for these enveloped viruses, as well as vesicular stomatitis virus. In contrast, we found no change in infectivity for Coxsackievirus B3, a non-enveloped virus. Derivatives of curcumin also exhibited antiviral activity against enveloped viruses. Further examination revealed that curcumin interfered with the binding of the enveloped viruses to cells in a dose-dependent manner, though the integrity of the viral RNA was maintained. Together, these results expand the family of viruses sensitive to curcumin and provide a mechanism of action for curcumin's effect on these enveloped viruses.
从香料和药草中提取的几种化合物在体外具有抗病毒作用，提示了其潜在的药理学用途。姜黄素是姜黄的一种成分，因其潜在的药用特性而被用作食品添加剂和草药补充剂。此前，姜黄素对包括登革热病毒和丙型肝炎病毒等多种病毒均表现出抗病毒特性。在此，我们描述了姜黄素对寨卡病毒和基孔肯雅病毒这两种蚊媒暴发病毒的抗病毒作用。两种病毒在高达5 μM姜黄素处理细胞时均有反应，且不影响细胞活力。我们观察到，用姜黄素直接处理病毒，对于这些包膜病毒以及水疱性口炎病毒，病毒的感染性呈剂量和时间依赖性降低。相比之下，我们发现无包膜病毒柯萨奇病毒B3的感染性没有变化。姜黄素的衍生物对包膜病毒也表现出抗病毒活性。进一步研究表明，姜黄素以剂量依赖性方式干扰包膜病毒与细胞的结合，不过病毒RNA的完整性得以维持。总之，这些结果扩展了对姜黄素敏感的病毒家族，并为姜黄素对这些包膜病毒作用的机制提供了依据。
