Yin Dongdong, Liu Yayun, Liu Wei, Wang Guijun, Yin Lei, Wang Jieru, Dai Yin, Shen Xuehuai, Zhao Ruihong, Zhan Kai, Pan Xiaocheng
Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Sciences, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province; Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Hefei 230031, PR China.
College of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, PR China.
Poult Sci. 2025 May 31;104(8):105381. doi: 10.1016/j.psj.2025.105381.
Duck Tembusu virus (DTMUV) is a significant etiological agent responsible for egg production decline in laying ducks and retarded growth in ducklings, contributing to considerable economic losses in the poultry sector. Current research mainly focuses on the development of vaccines for the prevention of DTMUV. However, there is relatively little research on antiviral drugs against this virus. Curcumin has been reported to exert antiviral effects against multiple viruses. However, its efficacy against DTMUV remains unclear. This study aimed to investigate the antiviral activity of curcumin against DTMUV both in vitro and in vivo. Cytotoxicity in BHK-21 cells was assessed utilizing the Cell Counting Kit-8 (CCK-8) assay. The antiviral effects of curcumin were evaluated using viral titration, qRT-PCR, Western blotting, and indirect immunofluorescence. In addition, a DTMUV-infected duck model was used to assess the therapeutic potential of curcumin in vivo. The experimental results indicated that curcumin was non-toxic to BHK-21 cells at concentrations up to 30 μM. In vitro, curcumin significantly reduced DTMUV replication in a dose-dependent manner, as confirmed by decreased viral titers, RNA copy numbers, and E protein expression. Further analysis revealed that curcumin exerted its antiviral effect primarily at the post-adsorption stage of the viral life cycle. In vivo, curcumin treatment improved the survival rate of DTMUV-infected ducklings and alleviated virus-associated pathology. These findings demonstrate that curcumin effectively inhibits DTMUV infection and highlight its potential as a viable candidate for the prevention and treatment of DTMUV-related diseases.
鸭坦布苏病毒（DTMUV）是导致蛋鸭产蛋量下降和雏鸭生长发育迟缓的重要病原体，给家禽业造成了巨大经济损失。目前的研究主要集中在开发预防DTMUV的疫苗。然而，针对这种病毒的抗病毒药物研究相对较少。姜黄素已被报道对多种病毒具有抗病毒作用。然而，其对DTMUV的疗效仍不清楚。本研究旨在探讨姜黄素在体外和体内对DTMUV的抗病毒活性。利用细胞计数试剂盒-8（CCK-8）法评估BHK-21细胞的细胞毒性。通过病毒滴定、qRT-PCR、蛋白质免疫印迹和间接免疫荧光评估姜黄素的抗病毒效果。此外，使用DTMUV感染的鸭模型评估姜黄素在体内的治疗潜力。实验结果表明，姜黄素在浓度高达30μM时对BHK-21细胞无毒。在体外，姜黄素以剂量依赖的方式显著降低DTMUV的复制，病毒滴度、RNA拷贝数和E蛋白表达的降低证实了这一点。进一步分析表明，姜黄素主要在病毒生命周期的吸附后阶段发挥其抗病毒作用。在体内，姜黄素治疗提高了DTMUV感染雏鸭的存活率，并减轻了病毒相关的病理变化。这些发现表明姜黄素能有效抑制DTMUV感染，并突出了其作为预防和治疗DTMUV相关疾病的可行候选药物的潜力。
