School of Pharmacy, National Defense Medical Center, Taipei, Taiwan, ROC; Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, ROC.
Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
Biochem Biophys Res Commun. 2019 Oct 22;518(4):732-738. doi: 10.1016/j.bbrc.2019.08.120. Epub 2019 Aug 28.
Zika virus (ZIKV) is an emerging vector-borne virus that is associated with severe congenital cerebral anomalies in fetuses and paralytic Guillain-Barré syndrome in adults. In the current global health crisis, there are no vaccines or therapeutics available for the treatment of ZIKV infection. In the present study, we evaluated the efficacy of the protoberberine alkaloid, palmatine, in inhibiting ZIKV and Japanese encephalitis virus (JEV). Palmatine was shown to bind to restricted viruses, inhibit ZIKV infection, and resist ZIKV-induced cytopathic effects. Palmatine was also shown to inhibit JEV infection in multiple cell lines. Overall, the effects of palmatine in disrupting ZIKV binding, entry, and stability indicate that this small molecule would be a good starting point for the development of treatments aimed at inhibiting ZIKV infection.
寨卡病毒（ZIKV）是一种新兴的虫媒病毒，与胎儿严重先天性脑异常和成人麻痹性格林-巴利综合征有关。在当前的全球卫生危机中，尚无针对 ZIKV 感染的疫苗或治疗方法。在本研究中，我们评估了原小檗碱生物碱黄连碱抑制寨卡病毒和日本脑炎病毒（JEV）的功效。研究表明，黄连碱可与受限病毒结合，抑制 ZIKV 感染并抵抗 ZIKV 诱导的细胞病变效应。黄连碱还可抑制多种细胞系中的 JEV 感染。总的来说，黄连碱破坏 ZIKV 结合、进入和稳定性的作用表明，这种小分子可能是开发旨在抑制 ZIKV 感染的治疗方法的良好起点。
