Yao Jun-Tao, Zhao Shu-Hong, Liu Qiu-Ping, Lv Mo-Qi, Zhou Dang-Xia, Liao Zi-Jun, Nan Ke-Jun
Department of Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China; The Integrated Department of TCM and Western Medicine, Affiliated Shaanxi Provincial Tumor Hospital of Medical College of Xi'an Jiaotong University, Shaanxi Provincial Tumor Hospital, Xi'an 710061, China.
Department of Radiation, Shaanxi Provinical People's Hospital, Xi'an 710068, China.
Pathol Res Pract. 2017 May;213(5):453-456. doi: 10.1016/j.prp.2017.02.011. Epub 2017 Feb 22.
Circular RNAs (circRNAs) are a novel type of endogenous RNAs featuring stable structure and high tissue-specific expression. Recently, accumulating evidence has revealed that aberrant circRNAs expression plays important roles in carcinogenesis and tumor progression. However, the expression pattern and biological function of circRNAs in non small cell lung cancer (NSCLC) remain largely unknown. Therefore, the purpose of this study was to clarify the possible role of one of typical circRNAs, circRNA_100876 in NSCLC and to define its prognostic value in NSCLC. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of circRNA_100876 in tumor tissues and their adjacent nontumorous tissues in 101 patients with NSCLC. We found that the expression level of circRNA_100876 was significantly elevated in NSCLC tissues when compared with their adjacent nontumorous tissues (P=0.000). Moreover, there was a close correlation between the circRNA_100876 up-regulation expression and lymph node metastasis (P=0.001) and tumor staging (P=0.001) in NSCLC. In addition, Kaplan-Meier survival analysis demonstrated that the overall survival time of NSCLC patients with high circRNA_100876 expression was significantly shorter than those patients with low circRNA_100876 expression (P=0.000). In conclusion, our findings indicate that circRNA_100876 is closely related to the carcinogenesis of NSCLC and it might be served as a potential prognostic biomarker and therapeutic target for NSCLC.
环状RNA（circRNAs）是一类新型的内源性RNA，具有稳定的结构和高度的组织特异性表达。最近，越来越多的证据表明，环状RNA表达异常在肿瘤发生和肿瘤进展中起重要作用。然而，环状RNA在非小细胞肺癌（NSCLC）中的表达模式和生物学功能仍 largely未知。因此，本研究的目的是阐明典型环状RNA之一circRNA_100876在NSCLC中的可能作用，并确定其在NSCLC中的预后价值。采用定量实时聚合酶链反应（qRT-PCR）检测101例NSCLC患者肿瘤组织及其癌旁非肿瘤组织中circRNA_100876的表达。我们发现，与癌旁非肿瘤组织相比，circRNA_100876在NSCLC组织中的表达水平显著升高（P = 0.000）。此外，circRNA_100876上调表达与NSCLC中的淋巴结转移（P = 0.001）和肿瘤分期（P = 0.001）密切相关。此外，Kaplan-Meier生存分析表明，circRNA_100876高表达的NSCLC患者的总生存时间明显短于circRNA_100876低表达的患者（P = 0.000）。总之，我们的研究结果表明，circRNA_100876与NSCLC的发生密切相关，它可能作为NSCLC的潜在预后生物标志物和治疗靶点。
