Solerte S B, Fioravanti M, Petraglia F, Facchinetti F, Patti A L, Schifino N, Genazzani A R, Ferrari E
Dipartimento di Medicina Interna, University of Pavia, Italy.
J Endocrinol Invest. 1988 Jan;11(1):37-42. doi: 10.1007/BF03350093.
A lipolytic activity for beta-endorphin (beta EP) has been recently suggested both in vitro and in vivo. In our study we evaluated the relationship between beta EP and blood lipid pattern in Type 2 (non-insulin dependent) diabetic patients. Plasma beta EP, together with plasma beta-lipotropin (beta LPH), ACTH, cortisol and plasma insulin (IRI), was measured by RIA after silicic acid plasma extraction and Sephedex G-75 column chromatography. Although reduced beta EP (7.12 +/- 3.8 fmol/ml) and increased beta LPH (9.3 +/- 3.7 fmol/ml) levels were found in diabetic patients, compared to controls (8.53 +/- 3.3 fmol/ml, p less than 0.05 and 8.34 +/- 2.6 fmol/ml, p less than 0.05, respectively), higher plasma beta EP concentrations were demonstrated in hyperlipidemic diabetic patients (10.3 +/- 3.9 fmol/ml) than in patients with normal blood lipid pattern (4.85 +/- 1.45 fmol/ml, p less than 0.001). Several positive correlations between beta EP, plasma free fatty acids (r = 0.75, p less than 0.001), triglycerides (r = 0.84, p less than 0.001) and VLDL (r = 0.80, p less than 0.001) were found in our patients independently of overweight, hypoglycemic treatment, plasma IRI levels and of the degree of metabolic control. A higher prevalence of micro- and macrovascular complications was demonstrated in hyperlipidemic than in normolipidemic patients. Blood lipid disorders might therefore be associated with increased plasma beta EP levels in Type 2 diabetes.
最近有研究表明，β-内啡肽（β-EP）在体外和体内均具有脂解活性。在我们的研究中，我们评估了2型（非胰岛素依赖型）糖尿病患者体内β-EP与血脂模式之间的关系。通过硅酸血浆萃取和葡聚糖凝胶G-75柱色谱法，采用放射免疫分析法（RIA）测定血浆β-EP，同时测定血浆β-促脂素（β-LPH）、促肾上腺皮质激素（ACTH）、皮质醇和血浆胰岛素（IRI）。与对照组相比（分别为8.53±3.3fmol/ml，p<0.05和8.34±2.6fmol/ml，p<0.05），糖尿病患者的β-EP水平降低（7.12±3.8fmol/ml），β-LPH水平升高（9.3±3.7fmol/ml），然而，高脂血症糖尿病患者的血浆β-EP浓度（10.3±3.9fmol/ml）高于血脂正常的患者（4.85±1.45fmol/ml，p<0.001）。在我们的患者中，发现β-EP与血浆游离脂肪酸（r=0.75，p<0.001）、甘油三酯（r=0.84，p<0.001）和极低密度脂蛋白（r=0.80，p<0.001）之间存在若干正相关，且与超重、降糖治疗、血浆IRI水平以及代谢控制程度无关。高脂血症患者的微血管和大血管并发症患病率高于血脂正常的患者。因此，血脂紊乱可能与2型糖尿病患者血浆β-EP水平升高有关。
