Biosciences, College of Dentistry, The Ohio State University, Columbus, OH, USA.
Sci Rep. 2017 Mar 27;7:45012. doi: 10.1038/srep45012.
Stressor exposure significantly affects the colonic mucosa-associated microbiota, and exacerbates Citrobacter rodentium-induced inflammation, effects that can be attenuated with probiotic Lactobacillus reuteri. This study assessed the structure of the colonic mucosa-associated microbiota in mice exposed to a social stressor (called social disruption), as well as non-stressed control mice, during challenge with the colonic pathogen C. rodentium. Mice were exposed to the social stressor or home cage control conditions for six consecutive days and all mice were challenged with C. rodentium immediately following the first exposure to the stressor. In addition, mice received probiotic L. reuteri, or vehicle as a control, via oral gavage following each stressor exposure. The stressor-exposed mice had significant differences in microbial community composition compared to non-stressed control mice. This difference was first evident following the six-cycle exposure to the stressor, on Day 6 post-C. rodentium challenge, and persisted for up to 19 days after stressor termination. Mice exposed to the stressor had different microbial community composition regardless of whether they were treated with L. reuteri or treated with vehicle as a control. These data indicate that stressor exposure affects the colonic microbiota during challenge with C. rodentium, and that these effects are long-lasting and not attenuated by probiotic L. reuteri.
应激暴露显著影响结肠黏膜相关微生物群，并加剧柠檬酸杆菌引起的炎症，而益生菌罗伊氏乳杆菌可以减轻这些影响。本研究评估了在结肠病原体柠檬酸杆菌感染期间，暴露于社会应激源（称为社交中断）的小鼠以及未暴露于应激源的对照小鼠的结肠黏膜相关微生物群结构。小鼠连续六天暴露于社会应激源或家庭笼对照条件下，所有小鼠在首次暴露于应激源后立即接受柠檬酸杆菌感染。此外，在每次应激暴露后，通过口服灌胃给予小鼠益生菌罗伊氏乳杆菌或载体作为对照。与未暴露于应激源的对照小鼠相比，应激暴露的小鼠的微生物群落组成存在显著差异。这种差异在应激源六周期暴露后的第 6 天（即柠檬酸杆菌感染后）首次出现，并在应激源结束后持续长达 19 天。无论是否用罗伊氏乳杆菌或用载体作为对照治疗，暴露于应激源的小鼠的微生物群落组成都不同。这些数据表明，应激暴露会影响柠檬酸杆菌感染期间的结肠微生物群，并且这些影响是持久的，益生菌罗伊氏乳杆菌不能减轻这些影响。
