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翻译起始因子eIF4E和eIFiso4E都是花生感染所必需的。

Translation Initiation Factor eIF4E and eIFiso4E Are Both Required for Infection in Peanut ( L.).

作者信息

Xu Manlin, Xie Hongfeng, Wu Juxiang, Xie Lianhui, Yang Jinguang, Chi Yucheng

机构信息

Shandong Peanut Research InstituteQingdao, China; Fujian Agriculture and Forestry UniversityFuzhou, China.

Shandong Peanut Research Institute Qingdao, China.

出版信息

Front Microbiol. 2017 Mar 10;8:338. doi: 10.3389/fmicb.2017.00338. eCollection 2017.

DOI:10.3389/fmicb.2017.00338
PMID:28344571
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5344889/
Abstract

(PStV) belongs to the genus and is the most important viral pathogen of cultivated peanut ( L.). The eukaryotic translation initiation factor, eIF4E, and its isoform, eIF(iso)4E, play key roles during virus infection in plants, particularly . In the present study, we cloned the and homologs in peanut and named these as and , respectively. Quantitative real-time PCR (qRT-PCR) analysis showed that these two genes were expressed during all growth periods and in all peanut organs, but were especially abundant in young leaves and roots. These also had similar expression levels. Yeast two-hybrid analysis showed that PStV multifunctional helper component proteinase (HC-Pro) and viral protein genome-linked (VPg) both interacted with PeaeIF4E and PeaeIF(iso)4E. Bimolecular fluorescence complementation assay showed that there was an interaction between HC-Pro and PeaeIF4E/PeaeIF(iso)4E in the cytoplasm and between VPg and PeaeIF4E/PeaeIF(iso)4E in the nucleus. Silencing either or using a virus-induced gene silencing system did not significantly affect PStV accumulation. However, silencing both and genes significantly weakened PStV accumulation. The findings of the present study suggest that PeaeIF4E and PeaeIF(iso)4E play important roles in the PStV infection cycle and may potentially contribute to PStV resistance.

摘要

花生条纹病毒（PStV）属于该属，是栽培花生（Arachis hypogaea L.）最重要的病毒病原体。真核翻译起始因子eIF4E及其异构体eIF(iso)4E在植物病毒感染过程中发挥关键作用，尤其是在……。在本研究中，我们克隆了花生中的eIF4E和eIF(iso)4E同源基因，并分别将它们命名为PeaeIF4E和PeaeIF(iso)4E。实时定量PCR（qRT-PCR）分析表明，这两个基因在花生的所有生长时期和所有器官中均有表达，但在幼叶和根中尤其丰富。它们也具有相似的表达水平。酵母双杂交分析表明，花生条纹病毒多功能辅助成分蛋白酶（HC-Pro）和病毒蛋白基因组连接蛋白（VPg）均与PeaeIF4E和PeaeIF(iso)4E相互作用。双分子荧光互补分析表明，HC-Pro与PeaeIF4E/PeaeIF(iso)4E在细胞质中相互作用，VPg与PeaeIF4E/PeaeIF(iso)4E在细胞核中相互作用。使用病毒诱导基因沉默系统沉默PeaeIF4E或PeaeIF(iso)4E均未显著影响花生条纹病毒的积累。然而，同时沉默这两个基因显著削弱了花生条纹病毒的积累。本研究结果表明，PeaeIF4E和PeaeIF(iso)4E在花生条纹病毒感染周期中发挥重要作用，并可能有助于花生对花生条纹病毒的抗性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4995/5344889/62d41c6405ff/fmicb-08-00338-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4995/5344889/713d5e3134be/fmicb-08-00338-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4995/5344889/c4bd2f97d01c/fmicb-08-00338-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4995/5344889/d1b9fd2c31ec/fmicb-08-00338-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4995/5344889/c6459b4d0347/fmicb-08-00338-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4995/5344889/433a1049bfaa/fmicb-08-00338-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4995/5344889/62d41c6405ff/fmicb-08-00338-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4995/5344889/713d5e3134be/fmicb-08-00338-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4995/5344889/c4bd2f97d01c/fmicb-08-00338-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4995/5344889/d1b9fd2c31ec/fmicb-08-00338-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4995/5344889/c6459b4d0347/fmicb-08-00338-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4995/5344889/433a1049bfaa/fmicb-08-00338-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4995/5344889/62d41c6405ff/fmicb-08-00338-g006.jpg

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