Ide K, Nishimura R, Kitajima T, Utsunomiya T, Tanabe K, Yuki Y, Mochizuki M
Department of Obstetrics and Gynecology, Kobe University School of Medicine.
Nihon Sanka Fujinka Gakkai Zasshi. 1988 Feb;40(2):160-6.
Molecular weights (MW) of urinary human chorionic gonadotropin (hCG) from trophoblastic diseases were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by Western blotting using specific antibodies. Although the MW of hCG beta from hydatidiform mole and invasive mole were identical to that of standard hCG beta, those from choriocarcinoma were individually different. These results indicate that the structural changes of carbohydrates, which may be induced by malignant transformation of trophoblast, can be reflected as differences in MW. In SDS-PAGE under nonreducing conditions. Western blotting using anti-hCG beta-carboxy-terminal peptides (CTP) revealed a single band corresponding to hCG beta. Under reducing conditions with dithiothreitol (DTT), however, a second low MW material (CTP') could be observed with anti-hCG beta-CTP. All hCG samples from invasive mole and choriocarcinoma were much more susceptible than standard hCG to release of CTP' by DTT. Estimation of MW and susceptibility to DTT reducing of urinary hCG by Western blotting are useful in the diagnosis of invasive mole and choriocarcinoma.
采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析滋养层疾病患者尿液中人绒毛膜促性腺激素（hCG）的分子量（MW），随后使用特异性抗体进行蛋白质印迹分析。虽然葡萄胎和侵袭性葡萄胎中hCGβ的MW与标准hCGβ相同，但绒毛膜癌中的hCGβ分子量各不相同。这些结果表明，滋养层恶性转化可能诱导的碳水化合物结构变化可反映为MW的差异。在非还原条件下进行SDS-PAGE时，使用抗hCGβ羧基末端肽（CTP）进行蛋白质印迹分析显示出一条与hCGβ相对应的条带。然而，在使用二硫苏糖醇（DTT）的还原条件下，使用抗hCGβ-CTP可观察到第二种低MW物质（CTP'）。侵袭性葡萄胎和绒毛膜癌的所有hCG样本比标准hCG更容易被DTT释放出CTP'。通过蛋白质印迹分析估计尿液hCG的MW以及对DTT还原的敏感性，对侵袭性葡萄胎和绒毛膜癌诊断有用。
