Mizuochi T, Nishimura R, Taniguchi T, Utsunomiya T, Mochizuki M, Derappe C, Kobata A
Jpn J Cancer Res. 1985 Aug;76(8):752-9.
Human chorionic gonadotropin (hCG) highly purified from the urine of patients with trophoblastic diseases (choriocarcinoma and hydatidiform mole) and from healthy pregnant women contains four asparagine-linked sugar chains in one molecule. Comparative studies of the sugar chains released by hydrazinolysis revealed that the structures of the sugar chains of choriocarcinoma hCGs are quite different from those of mole and normal hCGs. The carbohydrate structures of mole hCGs are the same as those of normal hCG, while all choriocarcinoma hCGs examined contain two triantennary, two unusual biantennary, and one monoantennary complex-type sugar chains which are not found in normal hCGs. The triantennary and unusual biantennary sugar chains contain the +/- NeuAc alpha 2----Gal beta 1----4GlcNAc beta 1----4Man alpha 1----3 group in common. The total amounts of fucosylated sugar chains in choriocarcinoma hCGs are twice those found in the hCGs of other groups. Therefore, the appearance of the N-acetylglucosaminyl transferase responsible for the formation of the GlcNAc beta 1----4Man alpha 1----3 group and the increase of the fucosyl transferase responsible for the formation of the Fuc alpha 1----6 GlcNAc----Asn group appear to be specific characteristics of malignant transformation of the trophoblast, and the structural changes of sugar chains could serve as useful markers for the diagnosis of choriocarcinoma.
从滋养层疾病（绒毛膜癌和葡萄胎）患者尿液以及健康孕妇尿液中高度纯化得到的人绒毛膜促性腺激素（hCG），一个分子中含有四条天冬酰胺连接的糖链。对肼解释放出的糖链进行的比较研究表明，绒毛膜癌hCG糖链的结构与葡萄胎和正常hCG的糖链结构有很大不同。葡萄胎hCG的碳水化合物结构与正常hCG相同，而所有检测的绒毛膜癌hCG均含有两条三聚天线型、两条异常二聚天线型和一条单聚天线型复合糖链，这些糖链在正常hCG中未发现。三聚天线型和异常二聚天线型糖链共同含有+/-NeuAcα2----Galβ1----4GlcNAcβ1----4Manα1----3基团。绒毛膜癌hCG中岩藻糖基化糖链的总量是其他组hCG中糖链总量的两倍。因此，负责形成GlcNAcβ1----4Manα1----3基团的N-乙酰葡糖胺基转移酶的出现以及负责形成Fucα1----6GlcNAc----Asn基团的岩藻糖基转移酶的增加似乎是滋养层恶性转化的特异性特征，糖链结构的变化可作为绒毛膜癌诊断的有用标志物。
