CAL-B催化L-天冬氨酸二乙酯向α-连接多肽的区域选择性本体聚合。
CAL-B catalyzed regioselective bulk polymerization of l-aspartic acid diethyl ester to α-linked polypeptides.
作者信息
Totsingan Filbert, Centore Robert, Gross Richard A
机构信息
Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute (RPI), 4005B Biotechnology Building, 110 Eighth Street, Troy, New York 12180, USA.
出版信息
Chem Commun (Camb). 2017 Apr 4;53(28):4030-4033. doi: 10.1039/c7cc01300k.
This paper reports that the bulk polymerization of l-aspartic acid diethyl ester catalyzed by immobilized CAL-B at 80 °C for 24 h gives primarily (∼95%) α-linked poly(l-aspartate) in 70% yield with DP = 50 and regioselectivity (α/β) = 94 : 6. Plots of log{[M]/[M]} vs. time and DPvs. conversion indicate that this polymerization proceeds in a controlled manner by a chain-growth mechanism up to 90% conversion. Thereafter, competition occurs between chain growth and step mechanisms.
本文报道，固定化的南极假丝酵母脂肪酶B（CAL-B）在80℃下催化L-天冬氨酸二乙酯本体聚合24小时，主要生成（约95%）α-连接的聚L-天冬氨酸，产率为70%，聚合度（DP） = 50，区域选择性（α/β） = 94∶6。log{[M]/[M]}对时间的曲线以及DP对转化率的曲线表明，该聚合反应通过链增长机理以可控方式进行，直至转化率达到90%。此后，链增长和逐步机理之间发生竞争。
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