Chandrasekhar R, Huang H M, Sun G Y
Sinclair Comparative Medicine Research Farm, University of Missouri, Columbia.
J Pharmacol Exp Ther. 1988 Apr;245(1):120-3.
The effects of acute ethanol administration on the polyphosphoinositide metabolism of rat brain cerebral cortex were examined. Intracerebral injections of [gamma-32P]ATP proved to be an effective in vivo method to prelabel brain phospholipids, especially the polyphosphoinositides. High acute doses of ethanol (8 or 6 g/kg b.wt.) administered by gavage significantly inhibited the breakdown of polyphosphoinositides as judged by an elevation in the concentration as well as the labeling of these compounds. Concomitantly, there was a significant reduction in the level of diacylglycerols. Low acute doses of ethanol (2 g/kg b.wt.) did not seem to have any effects on the basal levels or labeling of these compounds. The changes in polyphosphoinositide labeling due to ethanol intoxication were reverted to near control values when animals regained their righting reflex (approximately 4 hr). These studies demonstrate that, under normal conditions, polyphosphoinositides and diacylglycerols are maintained in a dynamic equilibrium and that acute doses of ethanol can suppress the signal transduction process and disturb this equilibrium.
研究了急性给予乙醇对大鼠大脑皮层多磷酸肌醇代谢的影响。脑内注射[γ-32P]ATP被证明是一种在体内预标记脑磷脂尤其是多磷酸肌醇的有效方法。通过灌胃给予高急性剂量的乙醇（8或6克/千克体重），根据这些化合物的浓度升高以及标记情况判断，显著抑制了多磷酸肌醇的分解。同时，二酰基甘油水平显著降低。低急性剂量的乙醇（2克/千克体重）似乎对这些化合物的基础水平或标记没有任何影响。当动物恢复翻正反射（约4小时）时，因乙醇中毒导致的多磷酸肌醇标记变化恢复到接近对照值。这些研究表明，在正常条件下，多磷酸肌醇和二酰基甘油保持动态平衡，急性剂量的乙醇可抑制信号转导过程并扰乱这种平衡。
