Institute of Plant Protection, Henan Academy of Agricultural Sciences, Key Laboratory of Crop Pest Control of Henan Province, Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Zhengzhou 450002, China.
Sci Rep. 2017 Mar 27;7:45188. doi: 10.1038/srep45188.
Light is an important environmental signal for most insects. The Oriental Armyworm, Mythimna separata, is a serious pest of cereal crops worldwide, and is highly sensitive to light signals during its developmental and reproductive stages. However, molecular biological studies of its response to light stress are scarce, and related genomic information is not available. In this study, we sequenced and de novo assembled the transcriptomes of M. separata exposed to four different light conditions: dark, white light (WL), UV light (UVL) and yellow light (YL). A total of 46,327 unigenes with an average size of 571 base pairs (bp) were obtained, among which 24,344 (52.55%) matched to public databases. The numbers of genes differentially expressed between dark vs WL, dark vs UVL, dark vs YL, and UVL vs YL were 12,012, 12,950, 14,855, and 13,504, respectively. These results suggest that light exposure altered gene expression patterns in M. separata. Putative genes involved in phototransduction-fly, phototransduction, circadian rhythm-fly, olfactory transduction, and taste transduction were identified. This study thus identified a series of candidate genes and pathways potentially related to light stress in M. separata.
光是大多数昆虫重要的环境信号。东方粘虫（Mythimna separata）是一种严重危害全球谷类作物的害虫，其在发育和生殖阶段对光信号高度敏感。然而，其对光胁迫反应的分子生物学研究很少，相关的基因组信息也不可用。在这项研究中，我们对暴露在四种不同光条件下的东方粘虫（Mythimna separata）进行了转录组测序和从头组装：黑暗、白光（WL）、紫外光（UVL）和黄 光（YL）。共获得了 46327 个平均大小为 571 个碱基对（bp）的 unigenes，其中 24344 个（52.55%）与公共数据库匹配。在黑暗与 WL、黑暗与 UVL、黑暗与 YL 以及 UVL 与 YL 之间差异表达的基因数量分别为 12012、12950、14855 和 13504。这些结果表明，光照改变了东方粘虫的基因表达模式。鉴定出了参与光转导-飞、光转导、昼夜节律-飞、嗅觉转导和味觉转导的假定基因。因此，本研究鉴定了一系列可能与东方粘虫光胁迫相关的候选基因和途径。
