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利用转录组分析鉴定甜菜夜蛾 Mythimna separata 中的候选化学感受基因。

Identification of candidate chemosensory genes in Mythimna separata by transcriptomic analysis.

机构信息

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

Department of Entomology, College of Plant Protection, Henan Agricultural University, Zhengzhou, 450002, China.

出版信息

BMC Genomics. 2018 Jul 4;19(1):518. doi: 10.1186/s12864-018-4898-0.

DOI:10.1186/s12864-018-4898-0
PMID:29973137
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6030794/
Abstract

BACKGROUND

The oriental armyworm, Mythimna separata, is an economically important and common Lepidopteran pest of cereal crops. Chemoreception plays a key role in insect life, such as foraging, oviposition site selection, and mating partners. To better understand the chemosensory mechanisms in M. separata, transcriptomic analysis of antennae, labial palps, and proboscises were conducted using next-generation sequencing technology to identify members of the major chemosensory related genes.

RESULTS

In this study, 62 putative odorant receptors (OR), 20 ionotropic receptors (IR), 16 gustatory receptors (GR), 38 odorant binding proteins (OBP), 26 chemosensory proteins (CSP), and 2 sensory neuron membrane proteins (SNMP) were identified in M. separata by bioinformatics analysis. Phylogenetic analysis of these candidate proteins was performed. Differentially expressed genes (DEGs) analysis was used to determine the expressions of all candidate chemosensory genes and then the expression profiles of the three families of receptor genes were confirmed by real-time quantitative RT-PCR (qPCR).

CONCLUSIONS

The important genes for chemoreception have now been identified in M. separata. This study will provide valuable information for further functional studies of chemoreception mechanisms in this important agricultural pest.

摘要

背景

东方粘虫，Mythimna separata，是一种重要的经济和常见的鳞翅目害虫，对谷类作物造成危害。化学生态学在昆虫生活中起着关键作用，例如觅食、产卵地点选择和交配伴侣。为了更好地了解 M. separata 的化学感觉机制，使用下一代测序技术对触角、唇瓣和喙进行转录组分析，以鉴定主要化学感觉相关基因的成员。

结果

在这项研究中，通过生物信息学分析在 M. separata 中鉴定了 62 个假定的气味受体（OR）、20 个离子型受体（IR）、16 个味觉受体（GR）、38 个气味结合蛋白（OBP）、26 个化学感觉蛋白（CSP）和 2 个感觉神经元膜蛋白（SNMP）。对这些候选蛋白进行了系统发育分析。差异表达基因（DEGs）分析用于确定所有候选化学感觉基因的表达，然后通过实时定量 RT-PCR（qPCR）确认了三个受体基因家族的表达谱。

结论

现已在 M. separata 中鉴定出重要的化学感觉基因。这项研究将为进一步研究这种重要农业害虫的化学感觉机制提供有价值的信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/0e59c10bee52/12864_2018_4898_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/10c6dd4cdbd6/12864_2018_4898_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/c8c0fca6431c/12864_2018_4898_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/f427b171bc64/12864_2018_4898_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/543feeb5fa99/12864_2018_4898_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/93afb9b99743/12864_2018_4898_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/3577d7b73391/12864_2018_4898_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/e63a2fc7d085/12864_2018_4898_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/133e09cbde02/12864_2018_4898_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/0e59c10bee52/12864_2018_4898_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/10c6dd4cdbd6/12864_2018_4898_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/c8c0fca6431c/12864_2018_4898_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/f427b171bc64/12864_2018_4898_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/543feeb5fa99/12864_2018_4898_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/93afb9b99743/12864_2018_4898_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/3577d7b73391/12864_2018_4898_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/e63a2fc7d085/12864_2018_4898_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/133e09cbde02/12864_2018_4898_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ac/6030794/0e59c10bee52/12864_2018_4898_Fig9_HTML.jpg

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