New Benzimidazole-1,2,4-Triazole Hybrid Compounds: Synthesis, Anticandidal Activity and Cytotoxicity Evaluation.

Owing to the growing need for antifungal agents, we synthesized a new series 2-((5-(4-(5-substituted-1-benzimidazol-2-yl)phenyl)-4-substituted-4-1,2,4-triazol-3-yl)thio)-1-(substitutedphenyl)ethan-1-one derivatives, which were tested against species. The synthesized compounds were characterized and elucidated by FT-IR, ¹H-NMR, C-NMR and HR-MS spectroscopies. The synthesized compounds were screened in vitro anticandidal activity against species by broth microdiluation methods. In vitro cytotoxic effects of the final compounds were determined by MTT assay. Microbiological studies revealed that compounds , , , , , , and possess a good antifungal profile. Compounds was the most active derivative and showed comparable antifungal activity to those of reference drugs ketoconazole and fluconazole. Cytotoxicity evaluation of compounds , , , , , and showed that compounds and were the least cytotoxic agents. Effects of these two compounds against ergosterol biosynthesis were observed by LC-MS-MS method, which is based on quantification of ergosterol level in . Compounds and inhibited ergosterol biosynthesis concentration dependently. A fluorescence microscopy study was performed to visualize effect of compound against at cellular level. It was determined that compound has a membrane damaging effect, which may be related with inhibition of biosynthesis of ergosterol.