Osei-Safo Dorcas, Dziwornu Godwin Akpeko, Appiah-Opong Regina, Chama Mary Anti, Tuffour Isaac, Waibel Reiner, Amewu Richard, Addae-Mensah Ivan
Chemistry Department, School of Physical and Mathematical Sciences, College of Basic and Applied Sciences, University of Ghana, P.O. Box LG 56, Legon, Accra, Ghana.
Present address: Department of Chemistry, University of Cape Town, Rondebosch 7701, South Africa..
Molecules. 2017 Mar 27;22(4):532. doi: 10.3390/molecules22040532.
As part of our search for bioactive compounds from the Dichapetalaceae, repeated chromatographic purification of the roots of a hitherto unexamined species, , led to the isolation of the newly occurring 7-hydroxydichapetalin P () and the known dichapetalins A () and X (). Also isolated were the known compounds friedelin-2,3-lactone (), friedelan-3-one (), friedelan-3β-ol () and pomolic (), as well as the dipeptide aurantiamide acetate (). The compounds were characterized by direct interpretation of their IR, 1D NMR and 2D NMR spectral data and by comparison of their physico-chemical data, including their chromatographic profiles, with the literature and authentic samples in our compound library for the genus . The compounds were assayed for their anti-proliferative activities against the human T-lymphocytic leukemia (Jurkat), acute promyelocytic leukemia (HL-60) and T-lymphoblast-like leukemia (CEM) cell lines. Overall, dichapetalin X showed the strongest (3.14 μM) and broadest cytotoxic activities against all the leukemic cell lines tested, exhibiting even stronger activities than the standard compound, curcumin.
作为我们从毒鼠子科植物中寻找生物活性化合物工作的一部分，对一种此前未被研究过的物种的根部进行反复色谱纯化，得到了新发现的7-羟基毒鼠子素P（）以及已知的毒鼠子素A（）和X（）。同时还分离出了已知化合物木栓酮-2,3-内酯（）、木栓烷-3-酮（）、木栓烷-3β-醇（）和坡模醇酸（），以及二肽aurantiamide acetate（）。这些化合物通过对其红外光谱、一维核磁共振谱和二维核磁共振谱数据的直接解析，并将其物理化学数据（包括色谱图）与文献以及我们化合物库中该属的标准样品进行比较来进行表征。对这些化合物针对人T淋巴细胞白血病（Jurkat）、急性早幼粒细胞白血病（HL-60）和T淋巴母细胞样白血病（CEM）细胞系的抗增殖活性进行了测定。总体而言，毒鼠子素X对所有测试的白血病细胞系显示出最强（3.14 μM）且最广泛的细胞毒性活性，甚至比标准化合物姜黄素表现出更强的活性。
