Physical Chemistry I, Department of Chemistry and Biology & Research Center of Micro and Nanochemistry and Engineering (Cμ), University of Siegen , 57076 Siegen, Germany.
Centre for Biomedical and Biomaterials Research, University of Mauritius , MSIRI Building, Réduit 80837, Mauritius.
Biomacromolecules. 2017 May 8;18(5):1563-1573. doi: 10.1021/acs.biomac.7b00150. Epub 2017 Apr 6.
Novel electrospun materials for bone tissue engineering were obtained by blending biodegradable polyhydroxybutyrate (PHB) or polyhydroxybutyrate valerate (PHBV) with the anionic sulfated polysaccharide κ-carrageenan (κ-CG) in varying ratios. In both systems, the two components phase separated as shown by FTIR, DSC and TGA. According to the contact angle data, κ-CG was localized preferentially at the fiber surface in PHBV/κ-CG blends in contrast to PHB/κ-CG, where the biopolymer was mostly found within the fiber. In contrast to the neat polyester fibers, the blends led to the formation of much smaller apatite crystals (800 nm vs 7 μm). According to the MTT assay, NIH3T3 cells grew in higher density on the blend mats in comparison to neat polyester mats. The osteogenic differentiation potential of the fibers was determined by SaOS-2 cell culture for 2 weeks. Alizarin red-S staining suggested an improved mineralization on the blend fibers. Thus, PHBV/κ-CG fibers resulted in more pronounced bioactive and osteogenic properties, including fast apatite-forming ability and deposition of nanosized apatite crystals.
通过将可生物降解的聚羟基丁酸酯（PHB）或聚羟基丁酸酯戊酸酯（PHBV）与阴离子硫酸化多糖κ-卡拉胶（κ-CG）以不同的比例混合，获得了用于骨组织工程的新型电纺材料。在这两个系统中，两种成分都如 FTIR、DSC 和 TGA 所示相分离。根据接触角数据，与 PHB/κ-CG 相比，κ-CG 在 PHBV/κ-CG 混合物中优先定位于纤维表面，而生物聚合物主要存在于纤维内。与纯聚酯纤维相比，混合物导致形成更小的磷灰石晶体（800nm 对 7μm）。根据 MTT 测定，与纯聚酯垫相比，NIH3T3 细胞在混合垫上以更高的密度生长。通过 SaOS-2 细胞培养 2 周来确定纤维的成骨分化潜力。茜素红-S 染色表明在混合纤维上有改善的矿化。因此，PHBV/κ-CG 纤维具有更明显的生物活性和成骨特性，包括快速形成磷灰石的能力和纳米级磷灰石晶体的沉积。
