Centre for Biomedical and Biomaterials Research (CBBR), MSIRI Building, University of Mauritius, Réduit, Mauritius; Physical Chemistry I and Research Center of Micro and Nanochemistry and Engineering (Cμ), Department of Chemistry and Biology, University of Siegen, 57076 Siegen, Germany.
Centre for Biomedical and Biomaterials Research (CBBR), MSIRI Building, University of Mauritius, Réduit, Mauritius.
Mater Sci Eng C Mater Biol Appl. 2017 Jul 1;76:13-24. doi: 10.1016/j.msec.2017.02.156. Epub 2017 Feb 28.
In this paper, the biomineralization potential and cellular response of novel blend films of the anionic sulfated polysaccharides kappa-carrageenan (KCG) and fucoidan (FUC) derived from seaweeds with semi-crystalline polyhydroxybutyrate (PHB) and polyhydroxybutyrate-co-valerate (PHBV), respectively, were analyzed. The incorporation of KCG and FUC into PHB and PHBV, which has been studied here for the first time, led to an overall decrease in crystallinity, enhanced surface hydrophilicity, reduced brittleness and faster degradation of the polymer blend films. All PHB/KCG, PHBV/KCG and PHBV/FUC films exhibited a two-stage mass loss profiles with pH stabilization. PHBV/KCG film showed the highest biomineralization activity due the presence of sulfate groups on the surface of the films. NIH3T3 cells attached and proliferated well on all blend films on account of enhanced surface hydrophilicity and improved flexibility. PHBV/KCG led to a promoted cellular activity compared to PHBV/FUC, presumably due to phase separation and higher amount of biopolymer on the film surface that was a consequence of the immiscibility of the polymers in the blend films.
本文分析了源自海藻的阴离子硫酸化多糖角叉菜胶（KCG）和褐藻胶（FUC）与半结晶性聚羟基丁酸酯（PHB）和聚羟基丁酸酯-共-戊酸酯（PHBV）的新型共混膜的生物矿化潜力和细胞反应。这是首次研究将 KCG 和 FUC 掺入 PHB 和 PHBV 中，这导致聚合物共混膜的结晶度总体降低、表面亲水性增强、脆性降低、降解速度加快。所有 PHB/KCG、PHBV/KCG 和 PHBV/FUC 薄膜均表现出具有 pH 稳定的两阶段质量损失曲线。由于薄膜表面存在硫酸根基团，PHBV/KCG 薄膜表现出最高的生物矿化活性。由于表面亲水性增强和柔韧性提高，NIH3T3 细胞在所有共混膜上均能很好地附着和增殖。与 PHBV/FUC 相比，PHBV/KCG 导致细胞活性增强，这可能是由于聚合物在共混膜中的不混溶性导致相分离和薄膜表面上生物聚合物的量增加所致。
