Cell Culture Laboratory, Postgraduate Program in Biotechnology, Centro Universitário UNIVATES, Av. Avelino Tallini, 171, CEP: 959000-000 Lajeado, Rio Grande do Sul, Brazil.
Cellular and Molecular Laboratory, Medical School, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, Brazil.
Biomed Pharmacother. 2017 May;89:1431-1441. doi: 10.1016/j.biopha.2017.03.040. Epub 2017 Mar 22.
The search for new bioactive substances derived from natural products is daily growing. Among biologically active products used in therapeutic approaches, essential oils are described with wide range of therapeutic and pharmacological potential. Plants from Myrtaceae family have the presence of essential oils in its composition. Calyptranthes grandifolia, Calyptranthes tricona and Myrciaria plinioides species belong to this family and are used by the local population. However, there are no reports in the literature describing relevant characteristics about the potential and possible activities of these species. The aim of this study was to identify the main compounds, evaluate the antioxidant potential and investigate, in RAW264.7 and CHO-K1 cells, the cytotoxic activity of the essential oils from the leaves of C. grandifolia, C. tricona and M. plinioides plants, in order to ensure their use. The compounds were identified by GC-MS, antioxidant activity was determined by DPPH method and cytotoxicity was assessed by the Alamar Blue method, at 48 and 72h. The main compounds found in the essential oils were sesquiterpenes. None of the essential oils have antioxidant potential. In cytotoxicity assays, the essential oils from the plants in analysis showed moderate activity in the proposed conditions. The alterations observed between the data provided by the essential oils in question, between different cell lines, may be associated with their composition, suggesting action of minor compounds. These results may suggest that the essential oils from C. grandifolia, C. tricona and M. plinioides have considerable potential to be explored. Future studies will be conducted to obtain more information about the action pathway and potential of the identified compounds.
从天然产物中寻找新的生物活性物质的研究每天都在增长。在用于治疗方法的生物活性产品中，精油被描述为具有广泛的治疗和药理学潜力。桃金娘科植物的组成中都存在精油。大花红厚壳、三叶红厚壳和桃金娘属植物属于桃金娘科，被当地居民使用。然而，文献中没有报道描述这些物种的潜在和可能的活动的相关特征。本研究的目的是鉴定主要化合物，评估抗氧化潜力，并在 RAW264.7 和 CHO-K1 细胞中研究大花红厚壳、三叶红厚壳和桃金娘属植物叶精油的细胞毒性活性，以确保其使用安全。通过 GC-MS 鉴定化合物，通过 DPPH 法测定抗氧化活性，通过 Alamar Blue 法在 48 和 72h 评估细胞毒性。精油中的主要化合物为倍半萜烯。三种精油均无抗氧化潜力。在细胞毒性试验中，在所分析的植物精油在提出的条件下显示出中等活性。在不同细胞系之间，所研究的精油提供的数据之间的变化可能与其组成有关，表明存在少量化合物的作用。这些结果可能表明，大花红厚壳、三叶红厚壳和桃金娘属植物的精油具有相当大的探索潜力。未来的研究将进行，以获得更多关于鉴定化合物的作用途径和潜力的信息。
