Kich Débora Mara, Bitencourt Shanna, Caye Bruna, Faleiro Dalana, Alves Celso, Silva Joana, Pinteus Susete, Mergener Michelle, Majolo Fernanda, Boligon Aline Augusti, Santos Roberto Christ Vianna, Pedrosa Rui, de Souza Claucia Fernanda Volken, Goettert Márcia Inês
Laboratório de Cultura de Células, Programa de Pós-graduação em Biotecnologia, Centro Universitário UNIVATES, Av. Avelino Talini 171, Lajeado, RS, 959000-000, Brazil.
Marine Resources Research Group (GIRM), ESTM, Polytechnic Institute of Leiria, 2520-641, Peniche, Portugal.
Mol Cell Biochem. 2017 Jan;424(1-2):35-43. doi: 10.1007/s11010-016-2840-9. Epub 2016 Oct 4.
Calyptranthes tricona is a species (Myrtaceae) native to South Brazil. Plants belonging to this family are folkloric used for analgesia, inflammation, and infectious diseases. However, little is known about the toxic potential of C. tricona. The present study aimed to evaluate the antioxidant activity of C. tricona ethanol and hexane leaf extracts, as well as verify their effect on human lymphocytes and MCF-7 cells. The extracts were subjected to preliminary phytochemical screening, antioxidant activity using DPPH and ORAC methods. Genotoxic and mutagenic effects in cultured human lymphocytes were assessed using the comet assay and the micronucleus assay, respectively. In addition, cell viability by MTT assay and fluorometric analysis of mitochondrial potential and caspases-9 activity were performed in order to verify the possible effects of both extracts on HO-induced cell death of MCF-7 cells. Our findings revealed that the phenol content and the antioxidant activity were only present in the ethanol extract. Also, the phytochemical screening presented steroids, triterpenoids, condensed tannins, and flavones as the main compounds. However, both extracts were capable of inducing concentration-dependent DNA damage in human lymphocytes. When treating MCF-7 cells with the extracts, both of them inhibited MCF-7 cell death in response to oxidative stress through a decrease of mitochondrial depolarization and caspases-9 activity. Thus, our results need to be considered in future in vitro and in vivo studies of C. tricona effects. In the meanwhile, we recommend caution in the acute/chronic use of this homemade preparation for medicinal purpose.
三角卡利普藤是一种原产于巴西南部的桃金娘科植物。该科植物在民间用于镇痛、抗炎和治疗传染病。然而，关于三角卡利普藤的潜在毒性知之甚少。本研究旨在评估三角卡利普藤乙醇和己烷叶提取物的抗氧化活性，并验证它们对人淋巴细胞和MCF - 7细胞的影响。对提取物进行了初步的植物化学筛选，采用DPPH和ORAC方法测定抗氧化活性。分别使用彗星试验和微核试验评估培养的人淋巴细胞中的遗传毒性和诱变效应。此外，通过MTT试验检测细胞活力，并对线粒体电位和半胱天冬酶 - 9活性进行荧光分析，以验证两种提取物对HO诱导的MCF - 7细胞死亡的可能影响。我们的研究结果表明，酚类含量和抗氧化活性仅存在于乙醇提取物中。植物化学筛选还表明，类固醇、三萜类化合物、缩合单宁和黄酮类是主要化合物。然而，两种提取物都能够在人淋巴细胞中诱导浓度依赖性的DNA损伤。当用提取物处理MCF - 7细胞时，它们都通过降低线粒体去极化和半胱天冬酶 - 9活性来抑制MCF - 7细胞因氧化应激而死亡。因此，在未来对三角卡利普藤作用的体外和体内研究中需要考虑我们的结果。同时，我们建议在将这种自制制剂用于药用的急性/慢性使用中要谨慎。
