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神经细胞黏附分子的多唾液酸单位在肾母细胞瘤中的重新表达。

Reexpression of poly(sialic acid) units of the neural cell adhesion molecule in Wilms tumor.

作者信息

Roth J, Zuber C, Wagner P, Taatjes D J, Weisgerber C, Heitz P U, Goridis C, Bitter-Suermann D

机构信息

Biocenter, University of Basle, Switzerland.

出版信息

Proc Natl Acad Sci U S A. 1988 May;85(9):2999-3003. doi: 10.1073/pnas.85.9.2999.

DOI:10.1073/pnas.85.9.2999
PMID:2834727
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC280130/
Abstract

A unique structural feature of the neural cell adhesion molecule N-CAM is the presence of homopolymers of alpha (2----8)-linked sialic acid units. We have used two specific probes for the detection of poly(sialic acid) in normal human kidney and Wilms tumor: a monoclonal antibody against meningococci group B capsular polysaccharide (homopolymers of alpha (2----8)-linked sialic acid units), which shows no crossreactivity with polynucleotides and denaturated DNA, and bacteriophage-induced endosialidases specifically hydrolyzing alpha (2----8)-linked poly(sialic acid) units. Additionally, for the detection of N-CAM, antibodies recognizing the polypeptide portion of the molecule and biotinylated antisense RNA transcribed from a cDNA clone for N-CAM were applied. Poly(sialic acid) was regionally detectable in human embryonic kidney but undetectable in normal adult kidney, as already reported for rat kidney. The malignant Wilms tumor, which is characterized by the presence of structural components resembling those found in embryonic kidney, reexpressed poly(sialic acid) units and showed positive immunostaining for the polypeptide portion of N-CAM. Immunoblot analysis of Wilms tumor as well as human embryonic kidney and brain with the monoclonal anti-poly(sialic acid) antibody revealed in each case the same high molecular mass broad band. In situ hybridization demonstrated the presence of mRNA for N-CAM in Wilms tumor. We conclude that poly(sialic acid), most probably present on N-CAM, is an oncodevelopmental antigen in human kidney.

摘要

神经细胞黏附分子N-CAM的一个独特结构特征是存在α(2→8)连接的唾液酸单元同聚物。我们使用了两种特异性探针来检测正常人肾脏和肾母细胞瘤中的聚唾液酸：一种针对B群脑膜炎球菌荚膜多糖（α(2→8)连接的唾液酸单元同聚物）的单克隆抗体，它与多核苷酸和变性DNA无交叉反应，以及能特异性水解α(2→8)连接的聚唾液酸单元的噬菌体诱导的内切唾液酸酶。此外，为了检测N-CAM，还应用了识别该分子多肽部分的抗体以及从N-CAM的cDNA克隆转录而来的生物素化反义RNA。聚唾液酸在人胚胎肾中可局部检测到，但在正常成人肾脏中未检测到，这与大鼠肾脏的情况已报道的一致。以存在类似于胚胎肾中发现的结构成分特征的恶性肾母细胞瘤，重新表达了聚唾液酸单元，并对N-CAM的多肽部分显示出阳性免疫染色。用单克隆抗聚唾液酸抗体对肾母细胞瘤以及人胚胎肾和脑进行免疫印迹分析，在每种情况下均显示出相同的高分子量宽带。原位杂交表明肾母细胞瘤中存在N-CAM的mRNA。我们得出结论，很可能存在于N-CAM上的聚唾液酸是人类肾脏中的一种肿瘤发生发育抗原。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8989/280130/d91e6d59d9c8/pnas00261-0131-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8989/280130/8126a9abe3d4/pnas00261-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8989/280130/78612328c955/pnas00261-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8989/280130/2dad120d7493/pnas00261-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8989/280130/d5aae9c25588/pnas00261-0131-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8989/280130/d91e6d59d9c8/pnas00261-0131-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8989/280130/8126a9abe3d4/pnas00261-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8989/280130/78612328c955/pnas00261-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8989/280130/2dad120d7493/pnas00261-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8989/280130/d5aae9c25588/pnas00261-0131-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8989/280130/d91e6d59d9c8/pnas00261-0131-c.jpg

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Reexpression of poly(sialic acid) units of the neural cell adhesion molecule in Wilms tumor.神经细胞黏附分子的多唾液酸单位在肾母细胞瘤中的重新表达。
Proc Natl Acad Sci U S A. 1988 May;85(9):2999-3003. doi: 10.1073/pnas.85.9.2999.
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Presence of the long chain form of polysialic acid of the neural cell adhesion molecule in Wilms' tumor. Identification of a cell adhesion molecule as an oncodevelopmental antigen and implications for tumor histogenesis.神经细胞黏附分子的长链形式多唾液酸在肾母细胞瘤中的存在。一种细胞黏附分子作为肿瘤发生发育抗原的鉴定及其对肿瘤组织发生的意义。
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本文引用的文献

1
Occurrence of unique polysialosyl carbohydrate units in glycoproteins of developing brain.发育中大脑糖蛋白中独特多唾液酸碳水化合物单元的出现。
J Biol Chem. 1982 Oct 25;257(20):11966-70.
2
Purification and macromolecular properties of a sialic acid-specific lectin from the slug Limax flavus.黄蛞蝓唾液酸特异性凝集素的纯化及大分子特性
J Biol Chem. 1982 Jul 10;257(13):7574-80.
3
Adult and embryonic mouse neural cell adhesion molecules have different binding properties.成年和胚胎小鼠神经细胞黏附分子具有不同的结合特性。
Oncotarget. 2022 Jan 10;13:73-89. doi: 10.18632/oncotarget.28167. eCollection 2022.
4
Antibody-Mediated Endocytosis of Polysialic Acid Enables Intracellular Delivery and Cytotoxicity of a Glycan-Directed Antibody-Drug Conjugate.抗体介导的唾液酸多糖内吞作用使糖基导向抗体药物偶联物能够实现细胞内递送和细胞毒性。
Cancer Res. 2019 Apr 15;79(8):1810-1821. doi: 10.1158/0008-5472.CAN-18-3119. Epub 2019 Feb 26.
5
Exploration of the Sialic Acid World.探索唾液酸世界。
Adv Carbohydr Chem Biochem. 2018;75:1-213. doi: 10.1016/bs.accb.2018.09.001. Epub 2018 Nov 28.
6
Cell Adhesion Molecules and Ubiquitination-Functions and Significance.细胞黏附分子与泛素化：功能与意义。
Biology (Basel). 2015 Dec 23;5(1):1. doi: 10.3390/biology5010001.
7
Promotion of cell migration by neural cell adhesion molecule (NCAM) is enhanced by PSA in a polysialyltransferase-specific manner.多唾液酸以多唾液酸转移酶特异性的方式增强神经细胞黏附分子（NCAM）对细胞迁移的促进作用。
PLoS One. 2015 Apr 17;10(4):e0124237. doi: 10.1371/journal.pone.0124237. eCollection 2015.
8
Polysialic acid enhances the migration and invasion of human cytotrophoblasts.聚唾液酸增强人滋养层细胞的迁移和侵袭。
Glycobiology. 2013 May;23(5):593-602. doi: 10.1093/glycob/cws162. Epub 2012 Dec 3.
9
Lectins for histochemical demonstration of glycans.用于糖链组织化学显示的凝集素。
Histochem Cell Biol. 2011 Aug;136(2):117-30. doi: 10.1007/s00418-011-0848-5. Epub 2011 Jul 31.
10
Sequences at the interface of the fifth immunoglobulin domain and first fibronectin type III repeat of the neural cell adhesion molecule are critical for its polysialylation.神经细胞黏附分子第五免疫球蛋白结构域和第一纤维连接蛋白 III 型重复序列交界处的序列对其多唾液酸化至关重要。
J Biol Chem. 2011 Feb 11;286(6):4525-34. doi: 10.1074/jbc.M110.200386. Epub 2010 Dec 3.
Nature. 1983;304(5924):347-9. doi: 10.1038/304347a0.
4
Occurrence of alpha 2-8 linked polysialosyl units in a neural cell adhesion molecule.神经细胞黏附分子中α2-8连接的多唾液酸基元的出现。
Biochem Biophys Res Commun. 1983 Apr 29;112(2):482-7. doi: 10.1016/0006-291x(83)91490-0.
5
Cell adhesion molecules.细胞黏附分子
Science. 1983 Feb 4;219(4584):450-7. doi: 10.1126/science.6823544.
6
Differences in the carbohydrate structures of neural cell-adhesion molecules from adult and embryonic chicken brains.成年鸡和胚胎鸡大脑中神经细胞黏附分子碳水化合物结构的差异。
J Biol Chem. 1982 Sep 25;257(18):11064-9.
7
Cancer metastasis. Organ colonization and the cell-surface properties of malignant cells.癌症转移。器官定植与恶性细胞的细胞表面特性。
Biochim Biophys Acta. 1982 Dec 21;695(2):113-76. doi: 10.1016/0304-419x(82)90020-8.
8
Kinetics of homophilic binding by embryonic and adult forms of the neural cell adhesion molecule.神经细胞黏附分子胚胎型和成年型的同源性结合动力学
Proc Natl Acad Sci U S A. 1983 Sep;80(18):5762-6. doi: 10.1073/pnas.80.18.5762.
9
Developmental biology of a neural cell adhesion molecule.一种神经细胞黏附分子的发育生物学
Nature. 1984;310(5978):549-54. doi: 10.1038/310549a0.
10
Light and electron microscopic demonstration of sialic acid residues with the lectin from Limax flavus: a cytochemical affinity technique with the use of fetuin-gold complexes.利用黄蛞蝓凝集素对唾液酸残基进行光镜和电镜显示：一种使用胎球蛋白-金复合物的细胞化学亲和技术。
J Histochem Cytochem. 1984 Nov;32(11):1167-76. doi: 10.1177/32.11.6208237.