基于鲁米诺和硅量子点之间电致化学发光共振能量转移的溶菌酶新型适体传感器。
A novel aptasensor for lysozyme based on electrogenerated chemiluminescence resonance energy transfer between luminol and silicon quantum dots.
机构信息
School of Chemistry and Chemical Engineering, Anhui University of Technology, Maanshan 243002, China; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
School of Chemistry and Chemical Engineering, Anhui University of Technology, Maanshan 243002, China.
出版信息
Biosens Bioelectron. 2017 Aug 15;94:530-535. doi: 10.1016/j.bios.2017.03.044. Epub 2017 Mar 22.
In the present work, electrogenerated chemiluminescence (ECL) of luminol was investigated in neutral condition at a gold electrode in the presence of silicon quantum dots (SiQDs). The results revealed that SiQDs can not only greatly enhance luminol ECL, but also act as energy acceptor to construct a novel ECL resonance energy transfer (ECL-RET) system with luminol. As a result, strong anodic ECL signal was obtained in neutral condition at the bare gold electrode, which is suitable for biosensing application. Lysozyme exhibited apparent inhibiting effect on the ECL-RET system, based on which an ECL aptasensor was fabricated for the sensitive detection of lysozyme. The proposed method showed high sensitivity, good selectivity, and wide linearity for the detection of lysozyme in the range of 5.0×10-5.0×10gmL with a detection limit of 5.8×10gmL (3σ). The results suggested that as-proposed luminol/SiQDs ECL biosensor will be promising in the detection enzyme.
在本工作中，研究了在中性条件下硅量子点（SiQDs）存在下金电极上鲁米诺的电致化学发光（ECL）。结果表明，SiQDs 不仅可以大大增强鲁米诺 ECL，还可以作为能量受体，与鲁米诺构建新型 ECL 共振能量转移（ECL-RET）体系。因此，在裸金电极的中性条件下获得了强阳极 ECL 信号，这适用于生物传感应用。溶菌酶对 ECL-RET 体系表现出明显的抑制作用，基于此，构建了用于灵敏检测溶菌酶的 ECL 适体传感器。该方法对溶菌酶的检测表现出高灵敏度、良好的选择性和宽线性范围，在 5.0×10-5.0×10gmL 范围内检测限为 5.8×10gmL（3σ）。结果表明，所提出的鲁米诺/SiQDs ECL 生物传感器将在检测酶方面具有广阔的应用前景。
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