School of Chemistry and Chemical Engineering, Hexian Development Institute of Chemical Industry, Anhui University of Technology, Maanshan 243002, China.
School of Chemistry and Chemical Engineering, Hexian Development Institute of Chemical Industry, Anhui University of Technology, Maanshan 243002, China.
Talanta. 2019 Jul 1;199:507-512. doi: 10.1016/j.talanta.2019.02.099. Epub 2019 Mar 2.
Black phosphorus quantum dots (BPQDs) can react with Ru(bpy) to generate strong anodic electrogenerated chemiluminescence (ECL). However, the instability and the lack of functional groups on BPQDs limit its further application in the fabrication of ECL biosensor. In the present work, uniform BPQDs-styrene-acrylamide (St-AAm) nanospheres (BSAN) are synthesized by encapsulating BPQDs into St-AAm copolymer nanospheres. Sufficient amount of BPQDs can be embedded into nanospheres, and react with Ru(bpy) to generate strong anodic ECL which is comparable to that of pure BPQDs. Amino group of polymer endows BPQDs the ability to connect with DNA, and can be used to fabricate ECL aptasensor for the sensitive detection of lysozyme. The proposed aptasensor shows high sensitivity, good selectivity and stability for the detection of lysozyme in the range of 0.1-100 pg mL with a detection limit of 0.029 pg mL (3σ). The proposed method reveals the promising ECL sensing application of BP nanomaterials in the detection of various proteins.
黑磷量子点（BPQDs）可以与 Ru(bpy) 反应产生强的阳极电致化学发光（ECL）。然而，BPQDs 的不稳定性和缺乏官能团限制了其在 ECL 生物传感器制造中的进一步应用。在本工作中，通过将 BPQDs 封装到 St-AAm 共聚物纳米球中，合成了均匀的 BPQDs-苯乙烯-丙烯酰胺（St-AAm）纳米球（BSAN）。可以将足够量的 BPQDs 嵌入纳米球中，并与 Ru(bpy) 反应产生强的阳极 ECL，其强度可与纯 BPQDs 相媲美。聚合物的氨基赋予 BPQDs 与 DNA 连接的能力，并可用于制备 ECL 适体传感器，用于灵敏检测溶菌酶。所提出的适体传感器对溶菌酶的检测具有高灵敏度、良好的选择性和稳定性，检测范围为 0.1-100 pg mL，检测限为 0.029 pg mL（3σ）。该方法揭示了 BP 纳米材料在检测各种蛋白质方面具有有前途的 ECL 传感应用。
