Leung Jennifer P, Wu Sherry, Chou Keng C, Signorell Ruth
Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC V6T 1Z1, Canada.
ETH Zurich, Laboratory for Physical Chemistry, Wolfgang-Pauli-Strasse 10, CH-8093, Zürich, Switzerland.
Nanomaterials (Basel). 2013 Jan 31;3(1):86-106. doi: 10.3390/nano3010086.
Specialized gold nanostructures are of interest for the development of alternative treatment methods in medicine. Photothermal therapy combined with gene therapy that supports hyperthermia is proposed as a novel multimodal treatment method for prostate cancer. In this work, photothermal therapy using small (<100 nm) gold nanoparticles and near-infrared (NIR) laser irradiation combined with gene therapy targeting heat shock protein (HSP) 27 was investigated. A series of nanoparticles: nanoshells, nanorods, core-corona nanoparticles and hollow nanoshells, were synthesized and examined to compare their properties and suitability as photothermal agents. cellular uptake studies of the nanoparticles into prostate cancer cell lines were performed using light scattering microscopy to provide three-dimensional (3D) imaging. Small gold nanoshells (40 nm) displayed the greatest cellular uptake of the nanoparticles studied and were used in photothermal studies. Photothermal treatment of the cancer cell lines with laser irradiation at 800 nm at 4 W on a spot size of 4 mm (FWHM) for 6 or 10 min resulted in an increase in temperature of ~12 °C and decrease in cell viability of up to 70%. However, studies combining photothermal therapy with gene therapy targeting HSP27 did not result in additional sensitization of the prostate cancer cells to hyperthermia.
特殊的金纳米结构在医学替代治疗方法的开发中备受关注。光热疗法与支持热疗的基因疗法相结合，被提议作为一种新型的前列腺癌多模态治疗方法。在这项工作中，研究了使用小尺寸（<100 nm）金纳米颗粒和近红外（NIR）激光照射的光热疗法与靶向热休克蛋白（HSP）27的基因疗法相结合的情况。合成并检测了一系列纳米颗粒：纳米壳、纳米棒、核-壳纳米颗粒和中空纳米壳，以比较它们作为光热剂的性质和适用性。使用光散射显微镜对纳米颗粒进入前列腺癌细胞系的细胞摄取进行研究，以提供三维（3D）成像。小尺寸金纳米壳（40 nm）在所研究的纳米颗粒中显示出最大的细胞摄取量，并用于光热研究。用800 nm激光以4 W的功率在4 mm（半高宽）光斑尺寸下对癌细胞系进行6或10分钟的光热处理，导致温度升高约12°C，细胞活力降低高达70%。然而，将光热疗法与靶向HSP27的基因疗法相结合的研究并未使前列腺癌细胞对热疗产生额外的敏感性。
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