胆囊置管术：一种新型的重症急性胆管炎小鼠模型。
Catheterization of the gallbladder: A novel mouse model of severe acute cholangitis.
作者信息
Yu Jian-Hua, Tang Hai-Jun, Zhang Wei-Guang, Zhu Zhi-Yang, Ruan Xin-Xian, Lu Bao-Chun
机构信息
Jian-Hua Yu, Hai-Jun Tang, Zhi-Yang Zhu, Xin-Xian Ruan, Bao-Chun Lu, Department of Hepatobiliary Surgery, Shaoxing People's Hospital, Shaoxing Hospital of Zhejiang University, Shaoxing 312000, Zhejiang Province, China.
出版信息
World J Gastroenterol. 2017 Mar 14;23(10):1771-1779. doi: 10.3748/wjg.v23.i10.1771.
AIM
To establish a severe acute cholangitis (SAC) model in mice.
METHODS
Cholecystic catheterization was performed under the condition of bile duct ligation (BDL). Trans-cholecystic injection of lipopolysaccharide (LPS) was defined as the SAC animal model. Sham operation group, intraperitoneal injection of LPS without BDL group, intraperitoneal injection of LPS with BDL group and trans-cholecystic injection of normal saline with BDL group were defined as control groups. The survival rates and tissue injuries in liver, lungs and kidney were evaluated.
RESULTS
Mice in the SAC group showed a time-dependent mortality and much more severe tissue injuries in liver, lungs and kidney, compared with other groups. However, relieving biliary obstruction could effectively reduce mortality and attenuate liver injury in the SAC mouse model.
CONCLUSION
Trans-cholecystic injection of LPS under the condition of biliary obstruction could establish a repeatable and reversible mouse model of SAC.
目的
在小鼠中建立重症急性胆管炎（SAC）模型。
方法
在胆管结扎（BDL）的条件下进行胆囊插管。经胆囊注射脂多糖（LPS）被定义为SAC动物模型。假手术组、未行BDL腹腔注射LPS组、行BDL腹腔注射LPS组和行BDL经胆囊注射生理盐水组被定义为对照组。评估存活率以及肝、肺和肾的组织损伤情况。
结果
与其他组相比，SAC组小鼠呈现出时间依赖性死亡率，且肝、肺和肾的组织损伤更为严重。然而，解除胆道梗阻可有效降低SAC小鼠模型的死亡率并减轻肝损伤。
结论
在胆道梗阻条件下经胆囊注射LPS可建立一种可重复且可逆的SAC小鼠模型。
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