Yang Jianhui, Lu Baochun
Department of Hepatobiliary Surgery, Shaoxing People's Hospital, Zhejiang University Shaoxing Hospital, Shaoxing 312000, China.
Iran J Basic Med Sci. 2015 Nov;18(11):1124-9.
This study aimed to establish a novel non-binding, reversible rat model of acute cholangitis of the severe type (ACST).
Twenty-six rats were randomly divided into the sham-operated group (n=13) and the ACST group (n=13). All rats were intubated with a modified catheter through the external jugular vein. The ACST model was established by ligation of the distal bile duct, placing one end of a modified catheter in the common bile duct, and then injecting lipopolysaccharides from the other end of the catheter and sealing it. The common bile duct pressure was measured before and at 0, 24, 48, and 72 hr after the model was established; similarly, the levels of serum total bilirubin (TBIL), alanine aminotransferase (ALT), and tumor necrosis factor (TNF)-α were measured at 0, 12, 24, and 48 hr after the model was established.
Pathological examination of liver tissues was carried out at 24 and 72 hr. The common bile duct pressure increased gradually after the operation. Serum levels of TBIL, ALT, and TNF-α in the ACST group progressively increased and were significantly higher than those in the sham-operated group, at each time point (P<0.05).
Obvious pathological changes were observed in the liver tissue of rats in the ACST group. This model appears to reflect the early course of human ACST and thus, can be used in postoperative experimental studies of ACST.
本研究旨在建立一种新型的非束缚性、可逆性重症急性胆管炎（ACST）大鼠模型。
将26只大鼠随机分为假手术组（n = 13）和ACST组（n = 13）。所有大鼠经颈外静脉插入改良导管。通过结扎胆总管远端、将改良导管一端置于胆总管内，然后从导管另一端注入脂多糖并封闭来建立ACST模型。在模型建立前及建立后0、24、48和72小时测量胆总管压力；同样，在模型建立后0、12、24和48小时测量血清总胆红素（TBIL）、丙氨酸转氨酶（ALT）和肿瘤坏死因子（TNF）-α水平。
在24和72小时对肝组织进行病理检查。术后胆总管压力逐渐升高。ACST组血清TBIL、ALT和TNF-α水平在各时间点均逐渐升高，且显著高于假手术组（P<0.05）。
ACST组大鼠肝组织出现明显病理变化。该模型似乎能反映人类ACST的早期病程，因此可用于ACST的术后实验研究。
