Whitaker-Dowling P, Youngner J S
Department of Microbiology, Biochemistry and Molecular Biology, School of Medicine, University of Pittsburgh, Pennsylvania 15261.
Virology. 1988 May;164(1):171-5. doi: 10.1016/0042-6822(88)90633-2.
When purified, [35S]methionine-labeled vesicular stomatitis virus (VSV) was exposed to ultraviolet light, an irradiation-induced change in the viral proteins was detected by SDS-polyacrylamide gel electrophoresis and immunoblotting. With dose of uv irradiation in the same range as that required to inactivate VSV leader RNA, a loss occurred in the bands corresponding to the L and NS proteins concomitant with the appearance of several new bands of radioactivity throughout the gel. This alteration of viral proteins correlated with the loss of ability of the virus to inhibit host macromolecular synthesis. In light of these results, the role that has been ascribed to the VSV leader RNA in VSV-mediated host shut-off needs to be reevaluated.
纯化后的[35S]甲硫氨酸标记的水疱性口炎病毒（VSV）经紫外线照射后，通过SDS-聚丙烯酰胺凝胶电泳和免疫印迹法检测到病毒蛋白的辐射诱导变化。当紫外线照射剂量与使VSV前导RNA失活所需剂量相当时，对应于L和NS蛋白的条带出现缺失，同时整个凝胶中出现几条新的放射性条带。病毒蛋白的这种改变与病毒抑制宿主大分子合成能力的丧失相关。鉴于这些结果，VSV前导RNA在VSV介导的宿主关闭中所起的作用需要重新评估。
