Stan E I, Ekimovskiĭ A P, Aleĭnik S I, Zhuravlev B V
Vopr Pitan. 1988 Jan-Feb(1):39-43.
Gel chromatography in columns with Sephadex G-50 and biogel P-2 was used to separate a number of fractions formed in k-casein of cow's milk after short-term treatment with swine trypsin. These fractions, similar to beta-casomorphin, are capable of reducing the respiration and heart rates in rabbits after intravenous injection in a dose of 100 micrograms/kg, that suggests their capacity for interaction with opiate receptors. Some fractions injected subcutaneously induce in starving animals an electroencephalographic picture of satiation, similar to that observed after cholecystokinin injections. Correlation of amino acid composition and physiologic activity has evidenced that cholecystokinin and opioid effects are caused by different peptides.
使用装有葡聚糖G - 50和生物凝胶P - 2的柱进行凝胶色谱法，以分离经猪胰蛋白酶短期处理后的牛乳κ-酪蛋白中形成的多个组分。这些类似于β-酪蛋白吗啡的组分，在以100微克/千克的剂量静脉注射后能够降低兔子的呼吸和心率，这表明它们具有与阿片受体相互作用的能力。一些皮下注射的组分在饥饿动物中诱发饱足的脑电图图像，类似于注射胆囊收缩素后观察到的图像。氨基酸组成与生理活性的相关性证明胆囊收缩素和阿片样作用是由不同的肽引起的。
