蛋氨酸调控的. 中 CmhR 调控网络的基因表达与特性研究。
Methionine-mediated gene expression and characterization of the CmhR regulon in .
机构信息
1Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747 AG, Groningen, The Netherlands.
2Department of Bioinformatics and Biotechnology, G C University, Faisalabad, Pakistan.
出版信息
Microb Genom. 2016 Oct 21;2(10):e000091. doi: 10.1099/mgen.0.000091. eCollection 2016 Oct.
This study investigated the transcriptomic response of D39 to methionine. Transcriptome comparison of the D39 wild-type grown in chemically defined medium with 0-10 mM methionine revealed the elevated expression of various genes/operons involved in methionine synthesis and transport (, , , , , , , , , and ). Furthermore, -galactosidase assays and quantitative RT-PCR studies demonstrated that the transcriptional regulator, CmhR (SPD-0588), acts as a transcriptional activator of the , , , , and genes. A putative regulatory site of CmhR was identified in the promoter region of CmhR-regulated genes and this CmhR site was further confirmed by promoter mutational experiments.
本研究探究了 D39 对蛋氨酸的转录组反应。将野生型 D39 在含有 0-10mM 蛋氨酸的化学定义培养基中培养，进行转录组比较，结果显示各种参与蛋氨酸合成和运输的基因/操纵子的表达水平升高（,,,,,,,,, 和 ）。此外，β-半乳糖苷酶测定和定量 RT-PCR 研究表明，转录调节因子 CmhR（SPD-0588）作为,,,, 和 基因的转录激活剂发挥作用。在 CmhR 调节基因的启动子区域鉴定到一个假定的 CmhR 调节位点，通过启动子突变实验进一步证实了该 CmhR 位点。
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