Chen Jing, Zhang Jianing, Zhu Longping, Qian Chunguo, Tian Hongru, Zhao Zhimin, Jin Lu, Yang Depo
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China.
Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou, China.
Front Microbiol. 2022 Mar 2;13:823845. doi: 10.3389/fmicb.2022.823845. eCollection 2022.
() is an anaerobic Gram-positive bacterium generally considered as a human skin commensal, but is also involved in different infections, such as and surgical infections. Although there are a variety of treatments, the side effects and the problem of bacterial drug resistance still limit their clinical usage. In this study, we found that essential oil (EO) distilled from fresh mature possessed promising antibacterial activity against In order to elucidate its potential mechanism, bacteriostatic activity test, Live/Dead kit assay, scanning electron microscope (SEM), transmission electron microscope (TEM), and metabolomics were employed. In addition, the content of adenosine triphosphate (ATP) in bacterium and the activities of key enzymes involved in critical metabolic pathways were detected using a variety of biochemical assays. The results showed that EO exhibited significant antibacterial activity against at a minimum inhibitory concentration (MIC) of 400 μg/mL and a minimum bactericidal concentration (MBC) of 800 μg/mL, and EO could destroy morphology and inhibit its growth. Moreover, results from our study showed that EO had a significant effect on the normal metabolism. In total, 86 metabolites were altered, and 34 metabolic pathways related to the carbohydrate metabolism, energy metabolism, amino acid metabolism, as well as cell wall and cell membrane synthesis were perturbed after EO administration. The synthesis of ATP in bacterial cells was also severely inhibited, and the activities of key enzymes of the glycolysis and Wood-Werkman cycle were significantly affected (Pyruvate Carboxylase, Malate Dehydrogenase and Pyruvate kinase activities were decreased, and Hexokinase was increased). Taken together, these results illustrated that the bacteriostatic effect of EO against by breaking the bacterial cell morphology and perturbing cell metabolism, including inhibition of key enzyme activity and ATP synthesis. The results from our study may shed new light on the discovery of novel drugs with more robust efficacy.
（）是一种厌氧革兰氏阳性菌，通常被认为是人体皮肤共生菌，但也会引发不同类型的感染，例如（此处原文缺失具体感染类型）和手术感染。尽管有多种治疗方法，但副作用和细菌耐药性问题仍然限制了它们的临床应用。在本研究中，我们发现从新鲜成熟的（此处原文缺失具体植物或物质）中提取的精油（EO）对（此处原文缺失具体细菌名称）具有良好的抗菌活性。为了阐明其潜在机制，我们采用了抑菌活性测试、死活细胞试剂盒检测、扫描电子显微镜（SEM）、透射电子显微镜（TEM）以及代谢组学方法。此外，还使用了多种生化检测方法来检测细菌中三磷酸腺苷（ATP）的含量以及关键代谢途径中关键酶的活性。结果表明，EO对（此处原文缺失具体细菌名称）具有显著的抗菌活性，最低抑菌浓度（MIC）为400μg/mL，最低杀菌浓度（MBC）为800μg/mL，并且EO能够破坏（此处原文缺失具体细菌名称）的形态并抑制其生长。此外，我们的研究结果表明，EO对（此处原文缺失具体细菌名称）的正常代谢有显著影响。总共86种代谢物发生了变化，并且在EO处理后，34条与碳水化合物代谢、能量代谢、氨基酸代谢以及细胞壁和细胞膜合成相关的代谢途径受到干扰。细菌细胞中ATP的合成也受到严重抑制，糖酵解和伍德-韦克曼循环关键酶的活性受到显著影响（丙酮酸羧化酶、苹果酸脱氢酶和丙酮酸激酶活性降低，己糖激酶活性增加）。综上所述，这些结果表明EO通过破坏细菌细胞形态和干扰细胞代谢，包括抑制关键酶活性和ATP合成，从而对（此处原文缺失具体细菌名称）产生抑菌作用。我们的研究结果可能为发现更有效新药提供新的思路。
