Soto César, Poza Cristian, Contreras David, Yáñez Jorge, Nacaratte Fallon, Toral M Inés
Departamento de Química Analítica e Inorgánica, Facultad de Ciencias Químicas, Universidad de Concepción, Concepción, Chile.
Laboratorio de Química Analítica, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
J Anal Methods Chem. 2017;2017:9812894. doi: 10.1155/2017/9812894. Epub 2017 Feb 28.
Amorolfine (AOF) is a compound with fungicide activity based on the dual inhibition of growth of the fungal cell membrane, the biosynthesis and accumulation of sterols, and the reduction of ergosterol. In this work a sensitive kinetic and spectrophotometric method for the AOF quantitation based on the AOF oxidation by means of KMnO at 30 min (fixed time), pH alkaline, and ionic strength controlled was developed. Measurements of changes in absorbance at 610 nm were used as criterion of the oxidation progress. In order to maximize the sensitivity, different experimental reaction parameters were carefully studied via factorial screening and optimized by multivariate method. The linearity, intraday, and interday assay precision and accuracy were determined. The absorbance-concentration plot corresponding to tap water spiked samples was rectilinear, over the range of 7.56 × 10-3.22 × 10 mol L, with detection and quantitation limits of 2.49 × 10 mol L and 7.56 × 10 mol L, respectively. The proposed method was successfully validated for the application of the determination of the drug in the spiked tap water samples and the percentage recoveries were 94.0-105.0%. The method is simple and does not require expensive instruments or complicated extraction steps of the reaction product.
阿莫罗芬（AOF）是一种具有杀真菌活性的化合物，它基于对真菌细胞膜生长、甾醇生物合成与积累以及麦角甾醇减少的双重抑制作用。在本研究中，开发了一种灵敏的动力学分光光度法，用于在30分钟（固定时间）、碱性pH和离子强度可控的条件下，基于高锰酸钾对AOF的氧化作用来定量AOF。在610nm处吸光度变化的测量用作氧化进程的判据。为了使灵敏度最大化，通过因子筛选仔细研究了不同的实验反应参数，并采用多变量方法进行了优化。测定了线性度、日内和日间测定精密度与准确度。加标自来水样品的吸光度-浓度曲线呈直线，在7.56×10⁻³.22×10⁻⁶mol·L⁻¹范围内，检测限和定量限分别为2.49×10⁻⁶mol·L⁻¹和7.56×10⁻⁶mol·L⁻¹。所提出的方法成功地验证了在加标自来水样品中药物测定的应用，回收率为94.0 - 105.0%。该方法简单，不需要昂贵的仪器或对反应产物进行复杂的提取步骤。
