Ganar Ketan, Das Moushumee, Raut Ashwin Ashok, Mishra Anamika, Kumar Sachin
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.
National Institute of High Security Animal Diseases, OIE reference Lab on Avian Influenza, Bhopal, MP, 462022, India.
Arch Virol. 2017 Jul;162(7):2169-2174. doi: 10.1007/s00705-017-3340-2. Epub 2017 Mar 27.
Pigeon paramyxovirus type 1 (PPMV-1) is an antigenic variant of avian paramyxovirus type 1 (APMV-1), which infects pigeons. The virus causes high morbidity and mortality, creating an alarming state for the poultry industry. The present work describes the molecular and pathogenic characterization of a PPMV-1 strain isolated from pigeon in Bhopal, India. Complete genome sequence analysis revealed a genome of 15,192 nucleotides encoding six genes organized in the order 3'-N-P-M-F-HN-L-5'. The fusion gene sequence analysis showed the presence of multiple basic amino acids R-R-Q-K-R-F at the cleavage site corresponding to pathogenic strains. The mean death time and intracerebral pathogenicity index values indicated a mesogenic nature for the PPMV-1 isolate. On phylogenetic analysis, the strain clustered with genotype VI viruses, including isolates from pigeon and dove. The Bhopal strain showed significant intra and inter-genotype evolutionary distance, suggesting the emergence of a new sub-genotype, VIj.
鸽I型副粘病毒（PPMV-1）是禽I型副粘病毒（APMV-1）的一种抗原变异体，可感染鸽子。该病毒导致高发病率和死亡率，给家禽业造成了令人担忧的状况。目前的工作描述了从印度博帕尔的鸽子中分离出的一株PPMV-1毒株的分子和致病性特征。全基因组序列分析显示，该病毒基因组为15192个核苷酸，编码六个基因，排列顺序为3'-N-P-M-F-HN-L-5'。融合基因序列分析表明，在与致病毒株相对应的裂解位点存在多个碱性氨基酸R-R-Q-K-R-F。平均死亡时间和脑内致病性指数值表明该PPMV-1分离株具有中等毒力特性。系统发育分析显示，该毒株与VI型病毒聚类，包括来自鸽子和斑鸠的分离株。博帕尔毒株显示出显著的基因型内和基因型间进化距离，表明出现了一个新的亚基因型VIj。
